TY - JOUR
T1 - Differential expression of TLR2 and TLR4 in α4β7-positive leukocytes of patients with axial spondyloarthritis
AU - Romero-López, José Pablo
AU - Gómez-Martínez, David
AU - Domínguez-López, María Lilia
AU - Jiménez-Zamudio, Luis
AU - Casasola-Vargas, Julio César
AU - Burgos-Vargas, Rubén
AU - García-Latorre, Ethel
N1 - Publisher Copyright:
© 2019 The Author(s) 2019. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - Objectives: Expression of α4β7 integrin can identify gut-homing immune cells. This study aimed to determine the expression of Toll-like receptor 2 (TLR2) and TLR4 in α4β7-positive leukocytes of patients with axial SpA (axSpA). Methods: We analysed the frequencies of α4β7-positive T cells, Tγδcells and monocytes in 14 patients with axSpA and 14 healthy controls, together with the expression of TLR2 and TLR4 by flow cytometry. Also, the concentration of faecal calprotectin was measured in all patients and controls. Results: We found significantly higher percentages of α4β7-positive T (P = 0.026) and Tγδcells (P = 0.0118) in the patients with axSpA than in controls; these cells showed differential expression of TLR2 and TLR4 when compared with α4β7-negative cells. Such differences were not correlated with disease activity or faecal calprotectin concentration. Conclusion: There is an increase in circulating α4β7-positive T and Tγδcells in patients with axSpA. These cells differentially express TLR2 and TLR4.
AB - Objectives: Expression of α4β7 integrin can identify gut-homing immune cells. This study aimed to determine the expression of Toll-like receptor 2 (TLR2) and TLR4 in α4β7-positive leukocytes of patients with axial SpA (axSpA). Methods: We analysed the frequencies of α4β7-positive T cells, Tγδcells and monocytes in 14 patients with axSpA and 14 healthy controls, together with the expression of TLR2 and TLR4 by flow cytometry. Also, the concentration of faecal calprotectin was measured in all patients and controls. Results: We found significantly higher percentages of α4β7-positive T (P = 0.026) and Tγδcells (P = 0.0118) in the patients with axSpA than in controls; these cells showed differential expression of TLR2 and TLR4 when compared with α4β7-negative cells. Such differences were not correlated with disease activity or faecal calprotectin concentration. Conclusion: There is an increase in circulating α4β7-positive T and Tγδcells in patients with axSpA. These cells differentially express TLR2 and TLR4.
KW - Axial spondyloarthritis
KW - T γδcells
KW - Toll-like receptors
KW - α4β7 integrin
UR - http://www.scopus.com/inward/record.url?scp=85082542532&partnerID=8YFLogxK
U2 - 10.1093/rheumatology/kez364
DO - 10.1093/rheumatology/kez364
M3 - Artículo
C2 - 31578573
SN - 1462-0324
VL - 59
SP - 879
EP - 888
JO - Rheumatology
JF - Rheumatology
IS - 4
ER -