Differential expression of dnaA and dosR genes among members of the Mycobacterium tuberculosis complex under oxic and hypoxic conditions

Claudia Badillo-López, Arturo González-Mejía, A. Cecilia Helguera-Repetto, Laura P. Salas-Rangel, Sandra Rivera-Gutiérrez, Jorge F. Cerna-Cortés, Jorge A. González-y-Merchand

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Abstract

Major differences regarding the pathology and host immune response of the Beijing and Canettii genotypes of Mycobacterium tuberculosis have been reported; however, studies on the genetic expression of these genotypes during in vitro dormancy are scarce. This study examined the expression of five cell-cycle-related genes and two dormancy-related genes in M. canettii, M. tuberculosis H37Rv, and M. tuberculosis Beijing during the Wayne model of dormancy. The results showed that under hypoxic conditions the three tuberculosis genotypes were able to transcribe genes involved in DNA replication and cellular division. In addition, dosR was found to be up-regulated in M. tuberculosis Beijing during the exponential growth phase but down-regulated under hypoxic conditions. In this genotype, the replication-related gene dnaA was also strongly down-regulated. These latter two findings suggest that, compared to M. tuberculosis H37Rv and M. canettii, the Beijing genotype has a lower capacity to synthesize dosR, hspX, and dnaA mRNAs during in vitro dormancy.
Original languageAmerican English
Pages (from-to)9-13
Number of pages7
JournalInternational Microbiology
DOIs
StatePublished - 1 Jan 2010

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Mycobacterium tuberculosis
Genotype
Genes
cdc Genes
DNA Replication
Tuberculosis
Pathology
Messenger RNA
Beijing
Growth
In Vitro Techniques

Cite this

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title = "Differential expression of dnaA and dosR genes among members of the Mycobacterium tuberculosis complex under oxic and hypoxic conditions",
abstract = "Major differences regarding the pathology and host immune response of the Beijing and Canettii genotypes of Mycobacterium tuberculosis have been reported; however, studies on the genetic expression of these genotypes during in vitro dormancy are scarce. This study examined the expression of five cell-cycle-related genes and two dormancy-related genes in M. canettii, M. tuberculosis H37Rv, and M. tuberculosis Beijing during the Wayne model of dormancy. The results showed that under hypoxic conditions the three tuberculosis genotypes were able to transcribe genes involved in DNA replication and cellular division. In addition, dosR was found to be up-regulated in M. tuberculosis Beijing during the exponential growth phase but down-regulated under hypoxic conditions. In this genotype, the replication-related gene dnaA was also strongly down-regulated. These latter two findings suggest that, compared to M. tuberculosis H37Rv and M. canettii, the Beijing genotype has a lower capacity to synthesize dosR, hspX, and dnaA mRNAs during in vitro dormancy.",
author = "Claudia Badillo-L{\'o}pez and Arturo Gonz{\'a}lez-Mej{\'i}a and Helguera-Repetto, {A. Cecilia} and Salas-Rangel, {Laura P.} and Sandra Rivera-Guti{\'e}rrez and Cerna-Cort{\'e}s, {Jorge F.} and Gonz{\'a}lez-y-Merchand, {Jorge A.}",
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Differential expression of dnaA and dosR genes among members of the Mycobacterium tuberculosis complex under oxic and hypoxic conditions. / Badillo-López, Claudia; González-Mejía, Arturo; Helguera-Repetto, A. Cecilia; Salas-Rangel, Laura P.; Rivera-Gutiérrez, Sandra; Cerna-Cortés, Jorge F.; González-y-Merchand, Jorge A.

In: International Microbiology, 01.01.2010, p. 9-13.

Research output: Contribution to journalArticle

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AU - Salas-Rangel, Laura P.

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AU - Cerna-Cortés, Jorge F.

AU - González-y-Merchand, Jorge A.

PY - 2010/1/1

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