Differential Detection of Enterovirus and Herpes Simplex Virus in Cerebrospinal Fluid by Real-Time RT-PCR

Brenda Sarquiz-Martínez, César R. González-Bonilla, Clara Esperanza Santacruz-Tinoco, José E. Munõz-Medina, Héctor D. Pardavé-Alejandre, Elizabeth Barbosa-Cabrera, José Ernesto Ramírez-González, José Alberto Diáz-Quinõnez

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Enterovirus (EV) and herpes simplex virus 1 and 2 (HSV1 and HSV2) are the main etiologic agents of central nervous system infections. Early laboratory confirmation of these infections is performed by viral culture of the cerebrospinal fluid (CSF), or the detection of specific antibodies in serum (e.g., HSV). The sensitivity of viral culture ranges from 65 to 75%, with a recovery time varying from 3 to 10 days. Serological tests are faster and easy to carry out, but they exhibit cross-reactivity between HSV1 and HSV2. Although molecular techniques are more sensitive (sensitivity >95%), they are more expensive and highly susceptible to cross-contamination. Methods: A real-time RT-PCR for the detection of EV, HSV1, and HSV2 was compared with end-point nested PCR. Results: We tested 87 CSF samples of patients with a clinical diagnosis of viral meningitis or encephalitis. Fourteen samples were found to be positive by RT-PCR, but only 8 were positive by end-point PCR. The RT-PCR showed a specificity range of 94-100%, the negative predictive value was 100%, and the positive predictive value was 62, 100, and 28% for HSV1, HSV2, and EV, respectively. Conclusion: Real-time RT-PCR detected EV, HSV1, and HSV2 with a higher sensitivity and specificity than end-point nested RT-PCR.

Original languageEnglish
Pages (from-to)118-124
Number of pages7
JournalIntervirology
Volume60
Issue number3
DOIs
StatePublished - 28 Sep 2017

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Enterovirus
Simplexvirus
Cerebrospinal Fluid
Real-Time Polymerase Chain Reaction
Polymerase Chain Reaction
Viral Meningitis
Laboratory Infection
Viral Encephalitis
Central Nervous System Infections
Human Herpesvirus 2
Human Herpesvirus 1
Serologic Tests
Sensitivity and Specificity
Antibodies
Serum

Keywords

  • Cerebrospinal fluid samples
  • Enterovirus
  • Herpes simplex virus
  • Real-time RT-PCR

Cite this

Sarquiz-Martínez, B., González-Bonilla, C. R., Santacruz-Tinoco, C. E., Munõz-Medina, J. E., Pardavé-Alejandre, H. D., Barbosa-Cabrera, E., ... Diáz-Quinõnez, J. A. (2017). Differential Detection of Enterovirus and Herpes Simplex Virus in Cerebrospinal Fluid by Real-Time RT-PCR. Intervirology, 60(3), 118-124. https://doi.org/10.1159/000480508
Sarquiz-Martínez, Brenda ; González-Bonilla, César R. ; Santacruz-Tinoco, Clara Esperanza ; Munõz-Medina, José E. ; Pardavé-Alejandre, Héctor D. ; Barbosa-Cabrera, Elizabeth ; Ramírez-González, José Ernesto ; Diáz-Quinõnez, José Alberto. / Differential Detection of Enterovirus and Herpes Simplex Virus in Cerebrospinal Fluid by Real-Time RT-PCR. In: Intervirology. 2017 ; Vol. 60, No. 3. pp. 118-124.
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Sarquiz-Martínez, B, González-Bonilla, CR, Santacruz-Tinoco, CE, Munõz-Medina, JE, Pardavé-Alejandre, HD, Barbosa-Cabrera, E, Ramírez-González, JE & Diáz-Quinõnez, JA 2017, 'Differential Detection of Enterovirus and Herpes Simplex Virus in Cerebrospinal Fluid by Real-Time RT-PCR', Intervirology, vol. 60, no. 3, pp. 118-124. https://doi.org/10.1159/000480508

Differential Detection of Enterovirus and Herpes Simplex Virus in Cerebrospinal Fluid by Real-Time RT-PCR. / Sarquiz-Martínez, Brenda; González-Bonilla, César R.; Santacruz-Tinoco, Clara Esperanza; Munõz-Medina, José E.; Pardavé-Alejandre, Héctor D.; Barbosa-Cabrera, Elizabeth; Ramírez-González, José Ernesto; Diáz-Quinõnez, José Alberto.

In: Intervirology, Vol. 60, No. 3, 28.09.2017, p. 118-124.

Research output: Contribution to journalArticle

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AU - Sarquiz-Martínez, Brenda

AU - González-Bonilla, César R.

AU - Santacruz-Tinoco, Clara Esperanza

AU - Munõz-Medina, José E.

AU - Pardavé-Alejandre, Héctor D.

AU - Barbosa-Cabrera, Elizabeth

AU - Ramírez-González, José Ernesto

AU - Diáz-Quinõnez, José Alberto

PY - 2017/9/28

Y1 - 2017/9/28

N2 - Background: Enterovirus (EV) and herpes simplex virus 1 and 2 (HSV1 and HSV2) are the main etiologic agents of central nervous system infections. Early laboratory confirmation of these infections is performed by viral culture of the cerebrospinal fluid (CSF), or the detection of specific antibodies in serum (e.g., HSV). The sensitivity of viral culture ranges from 65 to 75%, with a recovery time varying from 3 to 10 days. Serological tests are faster and easy to carry out, but they exhibit cross-reactivity between HSV1 and HSV2. Although molecular techniques are more sensitive (sensitivity >95%), they are more expensive and highly susceptible to cross-contamination. Methods: A real-time RT-PCR for the detection of EV, HSV1, and HSV2 was compared with end-point nested PCR. Results: We tested 87 CSF samples of patients with a clinical diagnosis of viral meningitis or encephalitis. Fourteen samples were found to be positive by RT-PCR, but only 8 were positive by end-point PCR. The RT-PCR showed a specificity range of 94-100%, the negative predictive value was 100%, and the positive predictive value was 62, 100, and 28% for HSV1, HSV2, and EV, respectively. Conclusion: Real-time RT-PCR detected EV, HSV1, and HSV2 with a higher sensitivity and specificity than end-point nested RT-PCR.

AB - Background: Enterovirus (EV) and herpes simplex virus 1 and 2 (HSV1 and HSV2) are the main etiologic agents of central nervous system infections. Early laboratory confirmation of these infections is performed by viral culture of the cerebrospinal fluid (CSF), or the detection of specific antibodies in serum (e.g., HSV). The sensitivity of viral culture ranges from 65 to 75%, with a recovery time varying from 3 to 10 days. Serological tests are faster and easy to carry out, but they exhibit cross-reactivity between HSV1 and HSV2. Although molecular techniques are more sensitive (sensitivity >95%), they are more expensive and highly susceptible to cross-contamination. Methods: A real-time RT-PCR for the detection of EV, HSV1, and HSV2 was compared with end-point nested PCR. Results: We tested 87 CSF samples of patients with a clinical diagnosis of viral meningitis or encephalitis. Fourteen samples were found to be positive by RT-PCR, but only 8 were positive by end-point PCR. The RT-PCR showed a specificity range of 94-100%, the negative predictive value was 100%, and the positive predictive value was 62, 100, and 28% for HSV1, HSV2, and EV, respectively. Conclusion: Real-time RT-PCR detected EV, HSV1, and HSV2 with a higher sensitivity and specificity than end-point nested RT-PCR.

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Sarquiz-Martínez B, González-Bonilla CR, Santacruz-Tinoco CE, Munõz-Medina JE, Pardavé-Alejandre HD, Barbosa-Cabrera E et al. Differential Detection of Enterovirus and Herpes Simplex Virus in Cerebrospinal Fluid by Real-Time RT-PCR. Intervirology. 2017 Sep 28;60(3):118-124. https://doi.org/10.1159/000480508