Differential activation of dendritic cells by Mycobacterium tuberculosis Beijing genotype

Juana Elizabeth Reyes-Martínez, Erik Nieto-Patlán, Alejandro Nieto-Patlán, Job Gonzaga-Bernachi, Teresa Santos-Mendoza, Jeanet Serafín-López, Alma Chávez-Blanco, Claudia Sandoval-Montes, Leopoldo Flores-Romo, Sergio Estrada-Parra, Iris Estrada-García, Rommel Chacón-Salinas

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Mycobacterium tuberculosis (Mtb) inhibits dendritric cells (DC) function in order to delay T cell response. Furthermore, there is increasing evidence that genetic diversity of Mtb strains can affect their interaction with the immune system. Beijing genotype has attracted attention because of its high prevalence and multi-drug resistance. Although it is known that this genotype is hypervirulent and differentially activates macrophages when compared to other genotypes, little is known about its interaction with DC. In order to address this issue, murine bone marrow derived DC (BMDC) were stimulated with soluble extracts (SE) from BCG, H37Rv, Canetti and Beijing genotypes. We observed that unlike other mycobacteria strains, SE-Beijing was unable to induce maturation of DC as assessed by cell surface MHC-II expression. DC stimulated with SE-Beijing failed to produce IL-12 and TNF-α, but did secrete IL-10. Interestingly, SE-Beijing induced CCR7 and PDL-1 on BMDC, but did not induce the expression of CD86. When BMDC stimulated with SE-Beijing were used to activate CD4+ cells they were unable to induce a Th1 response when compared with less virulent genotypes. These results indicate that Beijing is able to modulate DC activation and function, which may be related to the pathogenesis induced by this genotype.

Original languageEnglish
Pages (from-to)436-446
Number of pages11
JournalImmunological Investigations
Volume43
Issue number5
DOIs
StatePublished - 2014

Keywords

  • Beijing genotype
  • Cytokine production
  • Dendritic cells
  • Mycobacterium tuberculosis

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