Different expression of α and β mitochondrial estrogen receptors in the aging rat brain: Interaction with respiratory complex V

Carolina Álvarez-Delgado, C. Adriana Mendoza-Rodríguez, Ofir Picazo, Marco Cerbón

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Abstract

Recent evidence suggests that hormonal effects on mitochondria could be mediated by mitochondrial estrogen receptors (mtERs). These receptors are new candidates for the beneficial estrogenic effects on mitochondria in different physiological conditions. The aim of this investigation was to study mtER expression during brain aging. We analyzed mtERα and mtERβ expression in cortical, hippocampal and hypothalamic mitochondria of young adult (3. months) and aged (18. months) female Wistar rats by Western blot. In addition, we explored the interaction of mtERβ with respiratory complex V by using coimmunoprecipitation assays. The results show that mtERα and mtERβ are present in young and aged brain mitochondria. We also demonstrate that mtERs are expressed as variants and have a brain region specific distribution. The predominant mtER variants detected were of 61 and 55. KDa for mtERα and of 63 and 52. KDa for mtERβ. However, we did not observe differences in the mtERα or β content between the two age groups studied. Additionally, we show that mtERβ interacts with complex V.The overall results demonstrate that there is a differential expression of mtERα and mtERβ variants in different brain areas, indicating that they may participate in different functions in the brain during aging. © 2010 Elsevier Inc.
Original languageAmerican English
Pages (from-to)580-585
Number of pages521
JournalExperimental Gerontology
DOIs
StatePublished - 1 Jan 2010

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estrogens
Mitochondria
mitochondria
Estrogen Receptors
rats
brain
Rats
Brain
Aging of materials
interactions
Wistar Rats
Young Adult
Assays
Estrogens
Age Groups
Western Blotting
oligomycin sensitivity-conferring protein

Cite this

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title = "Different expression of α and β mitochondrial estrogen receptors in the aging rat brain: Interaction with respiratory complex V",
abstract = "Recent evidence suggests that hormonal effects on mitochondria could be mediated by mitochondrial estrogen receptors (mtERs). These receptors are new candidates for the beneficial estrogenic effects on mitochondria in different physiological conditions. The aim of this investigation was to study mtER expression during brain aging. We analyzed mtERα and mtERβ expression in cortical, hippocampal and hypothalamic mitochondria of young adult (3. months) and aged (18. months) female Wistar rats by Western blot. In addition, we explored the interaction of mtERβ with respiratory complex V by using coimmunoprecipitation assays. The results show that mtERα and mtERβ are present in young and aged brain mitochondria. We also demonstrate that mtERs are expressed as variants and have a brain region specific distribution. The predominant mtER variants detected were of 61 and 55. KDa for mtERα and of 63 and 52. KDa for mtERβ. However, we did not observe differences in the mtERα or β content between the two age groups studied. Additionally, we show that mtERβ interacts with complex V.The overall results demonstrate that there is a differential expression of mtERα and mtERβ variants in different brain areas, indicating that they may participate in different functions in the brain during aging. {\circledC} 2010 Elsevier Inc.",
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Different expression of α and β mitochondrial estrogen receptors in the aging rat brain: Interaction with respiratory complex V. / Álvarez-Delgado, Carolina; Mendoza-Rodríguez, C. Adriana; Picazo, Ofir; Cerbón, Marco.

In: Experimental Gerontology, 01.01.2010, p. 580-585.

Research output: Contribution to journalArticleResearchpeer-review

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