TY - JOUR
T1 - Diclofenac-enriched artificial sediment induces oxidative stress in Hyalella azteca
AU - Oviedo-Gómez, Dennis Gloria Carolina
AU - Galar-Martínez, Marcela
AU - García-Medina, Sandra
AU - Razo-Estrada, Celene
AU - Gómez-Oliván, Leobardo Manuel
N1 - Funding Information:
This study was made possible through support from the Research and Postgraduate Division of the National Polytechnic Institute, Mexico (SIP-IPN, Project 20080186).
PY - 2010/1
Y1 - 2010/1
N2 - Diclofenac is a nonsteroidal anti-inflammatory drug widely used in Mexico where it is sold over the counter. It enters water bodies through municipal and industrial discharges, posing a risk to water systems and aquatic organisms. Diclofenac-enriched artificial sediment was used to evaluate the toxicity of this pharmaceutical on the sentinel species Hyalella azteca, using oxidative stress biomarkers in order to determine if the set of tests used in this study is a suitable early damage biomarker. The median lethal concentration (72-h LC50) was determined and oxidative stress was evaluated using lipid peroxidation, protein carbonyl content to evaluate oxidized protein content, and the activity of superoxide dismutase, catalase, and glutathione peroxidase. All biomarkers were significantly altered. Diclofenac induces oxidative stress in H. azteca and the set of tests used (lipid peroxidation, protein carbonyl content, antioxidant enzyme activities) constitutes an adequate early damage biomarker for evaluating the toxicity of this pharmaceutical group in aquatic species.
AB - Diclofenac is a nonsteroidal anti-inflammatory drug widely used in Mexico where it is sold over the counter. It enters water bodies through municipal and industrial discharges, posing a risk to water systems and aquatic organisms. Diclofenac-enriched artificial sediment was used to evaluate the toxicity of this pharmaceutical on the sentinel species Hyalella azteca, using oxidative stress biomarkers in order to determine if the set of tests used in this study is a suitable early damage biomarker. The median lethal concentration (72-h LC50) was determined and oxidative stress was evaluated using lipid peroxidation, protein carbonyl content to evaluate oxidized protein content, and the activity of superoxide dismutase, catalase, and glutathione peroxidase. All biomarkers were significantly altered. Diclofenac induces oxidative stress in H. azteca and the set of tests used (lipid peroxidation, protein carbonyl content, antioxidant enzyme activities) constitutes an adequate early damage biomarker for evaluating the toxicity of this pharmaceutical group in aquatic species.
KW - Artificial sediment
KW - Diclofenac
KW - Hyalella azteca
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=72249099484&partnerID=8YFLogxK
U2 - 10.1016/j.etap.2009.09.004
DO - 10.1016/j.etap.2009.09.004
M3 - Artículo
SN - 1382-6689
VL - 29
SP - 39
EP - 43
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
IS - 1
ER -