TY - JOUR
T1 - Developmental changes in carnitine octanoyltransferase gene expression in intestine and liver of suckling rats
AU - Miliar, Angel
AU - Serra, Dolors
AU - Casaroli, Ricardo
AU - Vilaró, Senén
AU - Asins, Guillermina
AU - Hegardt, Fausto G.
N1 - Funding Information:
We thank Dr. B. Chatterjee for giving us the plasmid λ-2AxCOT. This work was supported by Grants PB95-0012 from the Comisión Interministerial de Ciencia y Tecnología, from the Fundació de la Marató de TV3 (1999), and 1999SGR0075 from the Generalitat of Catalunya, Spain. A.M. is recipient of fellowship from CONACYT (México). We also thank Mr. Robin Rycroft for his editorial help.
PY - 2001/1/15
Y1 - 2001/1/15
N2 - Carnitine octanoyltransferase (COT), which facilitates the transport of shortened fatty acyl-CoAs from peroxisomes to mitochondria, is expressed in the intestinal mucosa of suckling rats; its mRNA levels increase rapidly after birth, remain steady until day 15, and decrease until weaning, when basal, adult values are established, which remain unchanged thereafter. The process seems to be controlled at the transcriptional level since the developmental pattern of mRNA coincides with that of pre-mRNA values. Dam's milk may influence the intestinal expression of COT, since mRNA levels at birth are low and increase after the first lactation. Moreover, mRNA levels decrease in rats weaned on day 18 or 21. COT is also expressed in the liver of suckling rats. Hepatic COT mRNA is maximal at day 3, remains constant until day 9, and decreases thereafter; this pattern is also similar to that of pre-mRNA values. The profile of expression of COT in intestine and liver strongly resembles that of mitochondrial 3-hydroxy 3-methylglutaryl-coenzyme A synthase and carnitine palmitoyltransferase I, suggesting that analogous transcription factors modulate ketogenesis and mitochondrial and peroxisomal fatty acid oxidation.
AB - Carnitine octanoyltransferase (COT), which facilitates the transport of shortened fatty acyl-CoAs from peroxisomes to mitochondria, is expressed in the intestinal mucosa of suckling rats; its mRNA levels increase rapidly after birth, remain steady until day 15, and decrease until weaning, when basal, adult values are established, which remain unchanged thereafter. The process seems to be controlled at the transcriptional level since the developmental pattern of mRNA coincides with that of pre-mRNA values. Dam's milk may influence the intestinal expression of COT, since mRNA levels at birth are low and increase after the first lactation. Moreover, mRNA levels decrease in rats weaned on day 18 or 21. COT is also expressed in the liver of suckling rats. Hepatic COT mRNA is maximal at day 3, remains constant until day 9, and decreases thereafter; this pattern is also similar to that of pre-mRNA values. The profile of expression of COT in intestine and liver strongly resembles that of mitochondrial 3-hydroxy 3-methylglutaryl-coenzyme A synthase and carnitine palmitoyltransferase I, suggesting that analogous transcription factors modulate ketogenesis and mitochondrial and peroxisomal fatty acid oxidation.
KW - Carnitine octanoyltransferase
KW - Ketogenesis
KW - Peroxisomes
KW - Suckling rats
UR - http://www.scopus.com/inward/record.url?scp=0035862178&partnerID=8YFLogxK
U2 - 10.1006/abbi.2000.2155
DO - 10.1006/abbi.2000.2155
M3 - Artículo
SN - 0003-9861
VL - 385
SP - 283
EP - 289
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -