Development of DNA biosensors for single nucleotide polymorphisms

N. M. Pérez-Vielma, G. D. Ibarra-García, M. Gómez-López, M. A. Martínez Godínez, H. González Martínez, C. A. González Díaz, B. E. Del-Río-Navarro, V. F. Martínez-Silva, A. Martínez-Rivas, A. V. Vargas Varval, A. Miliar-García

Research output: Contribution to conferencePaper

Abstract

© Springer International Publishing Switzerland 2015. The development of a DNA biosensor for the detection of any single nucleotide polymorphism (SNP) implemented on the basis of a quartz crystal resonator as transducer is presented. The methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C SNPs were chosen to perform a proof of concept. We designed and proved a specific surface chemistry on gold in order to use human DNA with no chemical modification as probe. The immobilization of the human DNA via the proposed surface chemistry was successfully characterized with an Atomic Force Microscope (AFM). The DNA biosensor proposed here results in a specific and rapid manner to detect the evaluated SNP.
Original languageAmerican English
Pages55-58
Number of pages49
DOIs
StatePublished - 1 Jan 2015
EventIFMBE Proceedings -
Duration: 1 Jan 2016 → …

Conference

ConferenceIFMBE Proceedings
Period1/01/16 → …

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polymorphism
nucleotides
bioinstrumentation
deoxyribonucleic acid
chemistry
quartz crystals
immobilization
Switzerland
transducers
resonators
microscopes
gold
probes

Cite this

Pérez-Vielma, N. M. ; Ibarra-García, G. D. ; Gómez-López, M. ; Martínez Godínez, M. A. ; González Martínez, H. ; González Díaz, C. A. ; Del-Río-Navarro, B. E. ; Martínez-Silva, V. F. ; Martínez-Rivas, A. ; Vargas Varval, A. V. ; Miliar-García, A. / Development of DNA biosensors for single nucleotide polymorphisms. Paper presented at IFMBE Proceedings, .49 p.
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abstract = "{\circledC} Springer International Publishing Switzerland 2015. The development of a DNA biosensor for the detection of any single nucleotide polymorphism (SNP) implemented on the basis of a quartz crystal resonator as transducer is presented. The methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C SNPs were chosen to perform a proof of concept. We designed and proved a specific surface chemistry on gold in order to use human DNA with no chemical modification as probe. The immobilization of the human DNA via the proposed surface chemistry was successfully characterized with an Atomic Force Microscope (AFM). The DNA biosensor proposed here results in a specific and rapid manner to detect the evaluated SNP.",
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Pérez-Vielma, NM, Ibarra-García, GD, Gómez-López, M, Martínez Godínez, MA, González Martínez, H, González Díaz, CA, Del-Río-Navarro, BE, Martínez-Silva, VF, Martínez-Rivas, A, Vargas Varval, AV & Miliar-García, A 2015, 'Development of DNA biosensors for single nucleotide polymorphisms', Paper presented at IFMBE Proceedings, 1/01/16 pp. 55-58. https://doi.org/10.1007/978-3-319-13117-7_15

Development of DNA biosensors for single nucleotide polymorphisms. / Pérez-Vielma, N. M.; Ibarra-García, G. D.; Gómez-López, M.; Martínez Godínez, M. A.; González Martínez, H.; González Díaz, C. A.; Del-Río-Navarro, B. E.; Martínez-Silva, V. F.; Martínez-Rivas, A.; Vargas Varval, A. V.; Miliar-García, A.

2015. 55-58 Paper presented at IFMBE Proceedings, .

Research output: Contribution to conferencePaper

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AU - Pérez-Vielma, N. M.

AU - Ibarra-García, G. D.

AU - Gómez-López, M.

AU - Martínez Godínez, M. A.

AU - González Martínez, H.

AU - González Díaz, C. A.

AU - Del-Río-Navarro, B. E.

AU - Martínez-Silva, V. F.

AU - Martínez-Rivas, A.

AU - Vargas Varval, A. V.

AU - Miliar-García, A.

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