TY - JOUR
T1 - Development of digestive enzyme activity in spotted rose snapper, Lutjanus guttatus (Steindachner, 1869) larvae
AU - Moguel-Hernández, I.
AU - Peña, R.
AU - Nolasco-Soria, H.
AU - Dumas, S.
AU - Zavala-Leal, I.
N1 - Funding Information:
Acknowledgments The SIP-Instituto Politécnico Nacional project number 20121585 supported this study. I.M.H. is a recipient of PIFI-IPN and CONACYT doctoral fellowships. I.Z.L. is a recipient of a CONACYT postdoctoral fellowship. S.D. and R.P are COFAA-IPN and EDI-IPN fellows. The technical assistance of Patricia Hinojosa Baltazar is acknowledged.
PY - 2014/6
Y1 - 2014/6
N2 - We describe digestive enzyme activity during the larval development of spotted rose snapper, Lutjanus guttatus. Trypsin, chymotrypsin, leucine aminopeptidase, pepsin, amylase, lipase, and acid and alkaline phosphatase activities were evaluated using spectrophotometric techniques from hatching through 30 days. The spotted rose snapper larvae present the same pattern of digestive enzyme activity previously reported for other species in which pancreatic (i.e., trypsin, chymotrypsin, amylase, and lipase) and intestinal (i.e., acid and alkaline phosphatases and leucine aminopeptidase) enzymatic activities are present from hatching allowing the larvae to digest and absorb nutrients in the yolk-sac and live prey by the time of first feeding. The digestive and absorption capacity of the spotted rose snapper increases during the larval development. A significant increase in individual activity of all enzymes occurs at 20 DAH, and around 25 DAH, the juvenile-type of digestion is observed with the appearance of pepsin secreted by the stomach, suggesting that maturation of the digestive function occurs around 20-25 DAH. Our results are in agreement with a previous suggestion that early weaning may be possible from 20 DAH. However, the patterns of enzymatic activities reported in our study should be considered during the formulation of an artificial diet for early weaning of the spotted rose snapper.
AB - We describe digestive enzyme activity during the larval development of spotted rose snapper, Lutjanus guttatus. Trypsin, chymotrypsin, leucine aminopeptidase, pepsin, amylase, lipase, and acid and alkaline phosphatase activities were evaluated using spectrophotometric techniques from hatching through 30 days. The spotted rose snapper larvae present the same pattern of digestive enzyme activity previously reported for other species in which pancreatic (i.e., trypsin, chymotrypsin, amylase, and lipase) and intestinal (i.e., acid and alkaline phosphatases and leucine aminopeptidase) enzymatic activities are present from hatching allowing the larvae to digest and absorb nutrients in the yolk-sac and live prey by the time of first feeding. The digestive and absorption capacity of the spotted rose snapper increases during the larval development. A significant increase in individual activity of all enzymes occurs at 20 DAH, and around 25 DAH, the juvenile-type of digestion is observed with the appearance of pepsin secreted by the stomach, suggesting that maturation of the digestive function occurs around 20-25 DAH. Our results are in agreement with a previous suggestion that early weaning may be possible from 20 DAH. However, the patterns of enzymatic activities reported in our study should be considered during the formulation of an artificial diet for early weaning of the spotted rose snapper.
KW - Digestive function
KW - Enzyme activity
KW - Larvae development
KW - Lutjanusguttatus
KW - Spotted rose snapper
UR - http://www.scopus.com/inward/record.url?scp=84900330902&partnerID=8YFLogxK
U2 - 10.1007/s10695-013-9890-7
DO - 10.1007/s10695-013-9890-7
M3 - Artículo
SN - 0920-1742
VL - 40
SP - 839
EP - 848
JO - Fish Physiology and Biochemistry
JF - Fish Physiology and Biochemistry
IS - 3
ER -