Development of an immunobinding dot-blot assay as an alternative for the serodiagnosis of human cysticercosis

E. Hernandez-Cruz, J. J. González-Cabriales, C. Ordaz-Pichardo, N. I. De La Cruz-Hernández, G. H. Flores-Gutiérrez

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

An immunobinding dot-blot assay (DBA) was developed on nitrocellulose paper for the serodiagnosis of human cysticercosis, using Cysticercus cellulosae as antigen. The DBA had an immunological sensitivity of 0.08mg of antigen protein/ml; however, it showed cross-reactions with antigens of adult Taenia solium and Echinococcus granulosus, but not with Toxoplasma gondii and Entamoeba histolytica antigens. An enzyme-linked immunosorbent assay (ELISA) was used as the gold standard for obtaining the diagnostic validity of the DBA, giving 84.61%, 100.00%, 100.00% and 97.98% for epidemiological sensitivity, epidemiological specificity and positive and negative predictive values, respectively. There were no statistical differences between the two tests (P<0.05, kappa=0.907). This study showed that DBA is an alternative method for the serodiagnosis of human cysticercosis. Copyright © Cambridge University Press 2009.
Original languageAmerican English
Pages (from-to)333-337
Number of pages299
JournalJournal of Helminthology
DOIs
StatePublished - 1 Dec 2009
Externally publishedYes

Fingerprint

Cysticercosis
cysticercosis
serodiagnosis
Serologic Tests
antigens
Taenia solium
Antigens
assays
Cysticercus
Echinococcus granulosus
cellulose nitrate
Entamoeba histolytica
Collodion
sensitivity
Cross Reactions
Toxoplasma
Toxoplasma gondii
immunoassays
cross reaction
gold

Cite this

Hernandez-Cruz, E. ; González-Cabriales, J. J. ; Ordaz-Pichardo, C. ; De La Cruz-Hernández, N. I. ; Flores-Gutiérrez, G. H. / Development of an immunobinding dot-blot assay as an alternative for the serodiagnosis of human cysticercosis. In: Journal of Helminthology. 2009 ; pp. 333-337.
@article{5c0f798e7b78492fb5473a137335af2d,
title = "Development of an immunobinding dot-blot assay as an alternative for the serodiagnosis of human cysticercosis",
abstract = "An immunobinding dot-blot assay (DBA) was developed on nitrocellulose paper for the serodiagnosis of human cysticercosis, using Cysticercus cellulosae as antigen. The DBA had an immunological sensitivity of 0.08mg of antigen protein/ml; however, it showed cross-reactions with antigens of adult Taenia solium and Echinococcus granulosus, but not with Toxoplasma gondii and Entamoeba histolytica antigens. An enzyme-linked immunosorbent assay (ELISA) was used as the gold standard for obtaining the diagnostic validity of the DBA, giving 84.61{\%}, 100.00{\%}, 100.00{\%} and 97.98{\%} for epidemiological sensitivity, epidemiological specificity and positive and negative predictive values, respectively. There were no statistical differences between the two tests (P<0.05, kappa=0.907). This study showed that DBA is an alternative method for the serodiagnosis of human cysticercosis. Copyright {\circledC} Cambridge University Press 2009.",
author = "E. Hernandez-Cruz and Gonz{\'a}lez-Cabriales, {J. J.} and C. Ordaz-Pichardo and {De La Cruz-Hern{\'a}ndez}, {N. I.} and Flores-Guti{\'e}rrez, {G. H.}",
year = "2009",
month = "12",
day = "1",
doi = "10.1017/S0022149X09270866",
language = "American English",
pages = "333--337",
journal = "Journal of Helminthology",
issn = "0022-149X",
publisher = "Cambridge University Press",

}

Development of an immunobinding dot-blot assay as an alternative for the serodiagnosis of human cysticercosis. / Hernandez-Cruz, E.; González-Cabriales, J. J.; Ordaz-Pichardo, C.; De La Cruz-Hernández, N. I.; Flores-Gutiérrez, G. H.

In: Journal of Helminthology, 01.12.2009, p. 333-337.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Development of an immunobinding dot-blot assay as an alternative for the serodiagnosis of human cysticercosis

AU - Hernandez-Cruz, E.

AU - González-Cabriales, J. J.

AU - Ordaz-Pichardo, C.

AU - De La Cruz-Hernández, N. I.

AU - Flores-Gutiérrez, G. H.

PY - 2009/12/1

Y1 - 2009/12/1

N2 - An immunobinding dot-blot assay (DBA) was developed on nitrocellulose paper for the serodiagnosis of human cysticercosis, using Cysticercus cellulosae as antigen. The DBA had an immunological sensitivity of 0.08mg of antigen protein/ml; however, it showed cross-reactions with antigens of adult Taenia solium and Echinococcus granulosus, but not with Toxoplasma gondii and Entamoeba histolytica antigens. An enzyme-linked immunosorbent assay (ELISA) was used as the gold standard for obtaining the diagnostic validity of the DBA, giving 84.61%, 100.00%, 100.00% and 97.98% for epidemiological sensitivity, epidemiological specificity and positive and negative predictive values, respectively. There were no statistical differences between the two tests (P<0.05, kappa=0.907). This study showed that DBA is an alternative method for the serodiagnosis of human cysticercosis. Copyright © Cambridge University Press 2009.

AB - An immunobinding dot-blot assay (DBA) was developed on nitrocellulose paper for the serodiagnosis of human cysticercosis, using Cysticercus cellulosae as antigen. The DBA had an immunological sensitivity of 0.08mg of antigen protein/ml; however, it showed cross-reactions with antigens of adult Taenia solium and Echinococcus granulosus, but not with Toxoplasma gondii and Entamoeba histolytica antigens. An enzyme-linked immunosorbent assay (ELISA) was used as the gold standard for obtaining the diagnostic validity of the DBA, giving 84.61%, 100.00%, 100.00% and 97.98% for epidemiological sensitivity, epidemiological specificity and positive and negative predictive values, respectively. There were no statistical differences between the two tests (P<0.05, kappa=0.907). This study showed that DBA is an alternative method for the serodiagnosis of human cysticercosis. Copyright © Cambridge University Press 2009.

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=77949656390&origin=inward

UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=77949656390&origin=inward

U2 - 10.1017/S0022149X09270866

DO - 10.1017/S0022149X09270866

M3 - Article

SP - 333

EP - 337

JO - Journal of Helminthology

JF - Journal of Helminthology

SN - 0022-149X

ER -