Development of a spectrofluorometer with USB interface for in vivo measurements in surgical procedures

D. A. Fabila, J. M. De La Rosa, S. Stolik, E. Moreno, T. López, M. Álvarez, A. Arellano, G. De La Rosa, R. Mercado, J. L. Soto

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

In this paper we present the development of a portable spectrofluorometer with USB interface for in vivo measurements in biological tissue. The equipment consists of a mini-spectrometer HR4000CG-UV-NIR, with a working range from 200 to 1100 nm with a resolution of 0.5 nm FWHM. As the excitation source a light emitting diode at 365 nm, with maximum radiant power of 200 mW was used. A bifurcated fiber optic (R200-UV/VIS) with six 200 μm diameter irradiation fibers and one 200 μm collection fiber is used. The system is powered and controlled with a laptop, avoiding the use of an external power supply. A program to select the light source, to process and measure spectra in real time was developed using the graphic language G of LabVIEW 8.2. Also, in this issue are presented in vivo measurements of diffuse reflection and fluorescence of non-neoplastic and neoplastic cerebral tissue of patients affected with glioblastoma multiform tumors (GBM).

Original languageEnglish
Title of host publication2010 2nd Circuits and Systems for Medical and Environmental Applications Workshop, CASME 2010
DOIs
StatePublished - 2010
Event2010 2nd Circuits and Systems for Medical and Environmental Applications Workshop, CASME 2010 - Merida, Yucatan, Mexico
Duration: 13 Dec 201015 Dec 2010

Publication series

Name2010 2nd Circuits and Systems for Medical and Environmental Applications Workshop, CASME 2010

Conference

Conference2010 2nd Circuits and Systems for Medical and Environmental Applications Workshop, CASME 2010
Country/TerritoryMexico
CityMerida, Yucatan
Period13/12/1015/12/10

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