Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene

Luis Fernando Garcia-Melo, Miguel Morales-Rodríguez, Eduardo Madrigal-Bujaidar, Eduardo O. Madrigal-Santillán, José Antonio Morales-González, Rosa Natali Pineda Cruces, Jorge Alfredo Campoy Ramírez, Pablo Damian-Matsumura, Alexandro Tellez-Plancarte, Nikola Batina, Isela Álvarez-González

Research output: Contribution to journalArticlepeer-review

Abstract

In the scientific literature, it has been documented that electrochemical genosensors are novel analytical tools with proven clinical diagnostic potential for the identification of carcinogenic processes due to genetic and epigenetic alterations, as well as infectious diseases due to viruses or bacteria. In the present work, we describe the construction of an electrochemical genosensor for the identification of the k12p.1 mutation; it was based on use of Screen-Printed Gold Electrode (SPGE), Cyclic Voltammetry (CV), and Atomic Force Microscopy (AFM), for the monitoring the electron transfer trough the functionalized nanostructured surface and corresponding morphological changes. The sensitivity of the genosensor showed a linear response for the identification of the k12p.1 mutation of the K-ras gene in the concentration range of 10 fM to 1 μM with a detection limit of 7.96 fM in the presence of doxorubicin (Dox) as DNA intercalating agent and indicator of the hybridization reaction. Thus, the electrochemical genosensor developed could be useful for the identification of diseases related with the K-ras oncogene.

Original languageEnglish
Article number6575140
JournalJournal of Analytical Methods in Chemistry
Volume2022
DOIs
StatePublished - 2022

Fingerprint

Dive into the research topics of 'Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene'. Together they form a unique fingerprint.

Cite this