TY - JOUR
T1 - Development of a Nanostructured Electrochemical Genosensor for the Detection of the K-ras Gene
AU - Garcia-Melo, Luis Fernando
AU - Morales-Rodríguez, Miguel
AU - Madrigal-Bujaidar, Eduardo
AU - Madrigal-Santillán, Eduardo O.
AU - Morales-González, José Antonio
AU - Pineda Cruces, Rosa Natali
AU - Campoy Ramírez, Jorge Alfredo
AU - Damian-Matsumura, Pablo
AU - Tellez-Plancarte, Alexandro
AU - Batina, Nikola
AU - Álvarez-González, Isela
N1 - Publisher Copyright:
© 2022 Luis Fernando Garcia-Melo et al.
PY - 2022
Y1 - 2022
N2 - In the scientific literature, it has been documented that electrochemical genosensors are novel analytical tools with proven clinical diagnostic potential for the identification of carcinogenic processes due to genetic and epigenetic alterations, as well as infectious diseases due to viruses or bacteria. In the present work, we describe the construction of an electrochemical genosensor for the identification of the k12p.1 mutation; it was based on use of Screen-Printed Gold Electrode (SPGE), Cyclic Voltammetry (CV), and Atomic Force Microscopy (AFM), for the monitoring the electron transfer trough the functionalized nanostructured surface and corresponding morphological changes. The sensitivity of the genosensor showed a linear response for the identification of the k12p.1 mutation of the K-ras gene in the concentration range of 10 fM to 1 μM with a detection limit of 7.96 fM in the presence of doxorubicin (Dox) as DNA intercalating agent and indicator of the hybridization reaction. Thus, the electrochemical genosensor developed could be useful for the identification of diseases related with the K-ras oncogene.
AB - In the scientific literature, it has been documented that electrochemical genosensors are novel analytical tools with proven clinical diagnostic potential for the identification of carcinogenic processes due to genetic and epigenetic alterations, as well as infectious diseases due to viruses or bacteria. In the present work, we describe the construction of an electrochemical genosensor for the identification of the k12p.1 mutation; it was based on use of Screen-Printed Gold Electrode (SPGE), Cyclic Voltammetry (CV), and Atomic Force Microscopy (AFM), for the monitoring the electron transfer trough the functionalized nanostructured surface and corresponding morphological changes. The sensitivity of the genosensor showed a linear response for the identification of the k12p.1 mutation of the K-ras gene in the concentration range of 10 fM to 1 μM with a detection limit of 7.96 fM in the presence of doxorubicin (Dox) as DNA intercalating agent and indicator of the hybridization reaction. Thus, the electrochemical genosensor developed could be useful for the identification of diseases related with the K-ras oncogene.
UR - http://www.scopus.com/inward/record.url?scp=85141587807&partnerID=8YFLogxK
U2 - 10.1155/2022/6575140
DO - 10.1155/2022/6575140
M3 - Artículo
C2 - 36299712
AN - SCOPUS:85141587807
SN - 2090-8865
VL - 2022
JO - Journal of Analytical Methods in Chemistry
JF - Journal of Analytical Methods in Chemistry
M1 - 6575140
ER -