TY - JOUR
T1 - Development of 24 microsatellite markers for the white nosed coati (Nasua narica) using 454 sequencing
AU - Garibay, Eduardo Sánchez
AU - Silva-Caballero, Adrian
AU - Del Real-Monroy, Melina
AU - Lance, Stacey L.
AU - Valenzuela-Galván, David
AU - Ortega, Jorge
N1 - Funding Information:
Acknowledgments Financial support was provided by FOMIX-Campeche (CONACyT) 95900. Tissue samples from Nayarit were provided by C. López. We thank the field assistance provided by F. Reyes. Manuscript preparation was partially supported by the DOE under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation.
PY - 2012/9
Y1 - 2012/9
N2 - The White nosed coati (Nasua narica) it is a highly social carnivore inhabiting mainly Neotropical forests playing an important ecological role. A technique based on 454 sequencing of a library highly enriched for microsatellite repeats was used to develop loci for Nasua narica. Twenty-four polymorphic microsatellites were developed and tested as markers in the target species. All markers were genotyped on 18 different individuals from three distinct locations. We observed medium to low genetic variation across most loci (mean number of alleles per locus = 5). Levels of expected heterozygosity across all markers was fairly low (mean HE = 0.326, range 0.05-0.49).
AB - The White nosed coati (Nasua narica) it is a highly social carnivore inhabiting mainly Neotropical forests playing an important ecological role. A technique based on 454 sequencing of a library highly enriched for microsatellite repeats was used to develop loci for Nasua narica. Twenty-four polymorphic microsatellites were developed and tested as markers in the target species. All markers were genotyped on 18 different individuals from three distinct locations. We observed medium to low genetic variation across most loci (mean number of alleles per locus = 5). Levels of expected heterozygosity across all markers was fairly low (mean HE = 0.326, range 0.05-0.49).
KW - 454 Sequencing
KW - Expected heterozygosity
KW - Microsatellites
KW - Nasua narica
UR - http://www.scopus.com/inward/record.url?scp=84879547152&partnerID=8YFLogxK
U2 - 10.1007/s12686-012-9617-6
DO - 10.1007/s12686-012-9617-6
M3 - Artículo
SN - 1877-7252
VL - 4
SP - 661
EP - 663
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 3
ER -