Development and in vivo response of hydroxyapatite/whitlockite from chicken bones as bone substitute using a chitosan membrane for guided bone regeneration

Jorge Humberto Luna-Domínguez, Héctor Téllez-Jiménez, Heriberto Hernández-Cocoletzi, Margarita García-Hernández, José Aaron Melo-Banda, Håkan Nygren

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Biomaterials that meet the requirements to stimulate bone tissue formation play a vital role in orthopedics and dentistry. In this work, chitosan and a biphasic, non-cytotoxic material hydroxyapatite/whitlockite were obtained from natural sources, which are available as organic waste. The osteogenic activity was assessed using a rabbit model animal with a chitosan barrier membrane in combination with a bone-filling graft substitute composed of hydroxyapatite/whitlockite. FT-IR results showed the typical absorption bands of the chitosan and hydroxyapatite. Moreover, the X-ray diffraction pattern revealed a typical hexagonal phase of hydroxyapatite and rhombohedral structures related to whitlockite. Masson's trichrome stain showed an early formation of extracellular matrix mineralized, in accord with the surface morphology of a cortical mature bond observed by Scanning Electron Microscopy. The immunocytochemistry results showed a significant increase of positive immunoreactive cells to osteonectin in the treated defects in comparison with the control defects 6 and 8 weeks postoperatively. Overall, the results confirm that the use of this low-cost and versatile biomaterial as a barrier membrane and a bone substitute graft are useful for bone tissue engineering.

Original languageEnglish
Pages (from-to)22583-22591
Number of pages9
JournalCeramics International
Volume44
Issue number18
DOIs
StatePublished - 15 Dec 2018
Externally publishedYes

Fingerprint

Bone Substitutes
Chitosan
Durapatite
Hydroxyapatite
Bone
Membranes
Biocompatible Materials
Biomaterials
Grafts
Osteonectin
Dentistry
Defects
Orthopedics
Tissue engineering
Diffraction patterns
Surface morphology
Absorption spectra
Animals
Tissue
X ray diffraction

Keywords

  • Chitosan
  • Guided bone regeneration
  • Hydroxyapatite
  • Waste sources
  • Whitlockite

Cite this

Luna-Domínguez, Jorge Humberto ; Téllez-Jiménez, Héctor ; Hernández-Cocoletzi, Heriberto ; García-Hernández, Margarita ; Melo-Banda, José Aaron ; Nygren, Håkan. / Development and in vivo response of hydroxyapatite/whitlockite from chicken bones as bone substitute using a chitosan membrane for guided bone regeneration. In: Ceramics International. 2018 ; Vol. 44, No. 18. pp. 22583-22591.
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Development and in vivo response of hydroxyapatite/whitlockite from chicken bones as bone substitute using a chitosan membrane for guided bone regeneration. / Luna-Domínguez, Jorge Humberto; Téllez-Jiménez, Héctor; Hernández-Cocoletzi, Heriberto; García-Hernández, Margarita; Melo-Banda, José Aaron; Nygren, Håkan.

In: Ceramics International, Vol. 44, No. 18, 15.12.2018, p. 22583-22591.

Research output: Contribution to journalArticle

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AU - Melo-Banda, José Aaron

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