TY - JOUR
T1 - Development and in vivo response of hydroxyapatite/whitlockite from chicken bones as bone substitute using a chitosan membrane for guided bone regeneration
AU - Luna-Domínguez, Jorge Humberto
AU - Téllez-Jiménez, Héctor
AU - Hernández-Cocoletzi, Heriberto
AU - García-Hernández, Margarita
AU - Melo-Banda, José Aaron
AU - Nygren, Håkan
N1 - Publisher Copyright:
© 2018 Elsevier Ltd and Techna Group S.r.l.
PY - 2018/12/15
Y1 - 2018/12/15
N2 - Biomaterials that meet the requirements to stimulate bone tissue formation play a vital role in orthopedics and dentistry. In this work, chitosan and a biphasic, non-cytotoxic material hydroxyapatite/whitlockite were obtained from natural sources, which are available as organic waste. The osteogenic activity was assessed using a rabbit model animal with a chitosan barrier membrane in combination with a bone-filling graft substitute composed of hydroxyapatite/whitlockite. FT-IR results showed the typical absorption bands of the chitosan and hydroxyapatite. Moreover, the X-ray diffraction pattern revealed a typical hexagonal phase of hydroxyapatite and rhombohedral structures related to whitlockite. Masson's trichrome stain showed an early formation of extracellular matrix mineralized, in accord with the surface morphology of a cortical mature bond observed by Scanning Electron Microscopy. The immunocytochemistry results showed a significant increase of positive immunoreactive cells to osteonectin in the treated defects in comparison with the control defects 6 and 8 weeks postoperatively. Overall, the results confirm that the use of this low-cost and versatile biomaterial as a barrier membrane and a bone substitute graft are useful for bone tissue engineering.
AB - Biomaterials that meet the requirements to stimulate bone tissue formation play a vital role in orthopedics and dentistry. In this work, chitosan and a biphasic, non-cytotoxic material hydroxyapatite/whitlockite were obtained from natural sources, which are available as organic waste. The osteogenic activity was assessed using a rabbit model animal with a chitosan barrier membrane in combination with a bone-filling graft substitute composed of hydroxyapatite/whitlockite. FT-IR results showed the typical absorption bands of the chitosan and hydroxyapatite. Moreover, the X-ray diffraction pattern revealed a typical hexagonal phase of hydroxyapatite and rhombohedral structures related to whitlockite. Masson's trichrome stain showed an early formation of extracellular matrix mineralized, in accord with the surface morphology of a cortical mature bond observed by Scanning Electron Microscopy. The immunocytochemistry results showed a significant increase of positive immunoreactive cells to osteonectin in the treated defects in comparison with the control defects 6 and 8 weeks postoperatively. Overall, the results confirm that the use of this low-cost and versatile biomaterial as a barrier membrane and a bone substitute graft are useful for bone tissue engineering.
KW - Chitosan
KW - Guided bone regeneration
KW - Hydroxyapatite
KW - Waste sources
KW - Whitlockite
UR - http://www.scopus.com/inward/record.url?scp=85052986254&partnerID=8YFLogxK
U2 - 10.1016/j.ceramint.2018.09.032
DO - 10.1016/j.ceramint.2018.09.032
M3 - Artículo
AN - SCOPUS:85052986254
SN - 0272-8842
VL - 44
SP - 22583
EP - 22591
JO - Ceramics International
JF - Ceramics International
IS - 18
ER -