TY - JOUR
T1 - Determination of Trichinella spiralis in pig muscles using Mid-Fourier Transform Infrared Spectroscopy (MID-FTIR) with Attenuated Total Reflectance (ATR) and Soft Independent Modeling of Class Analogy (SIMCA)
AU - Gómez-De-Anda, Fabián
AU - Dorantes-Álvarez, Lidia
AU - Gallardo-Velázquez, Tzayhri
AU - Osorio-Revilla, Guillermo
AU - Calderón-Domínguez, Georgina
AU - Martínez Labat, Pablo
AU - de-la-Rosa-Arana, Jorge Luis
PY - 2012/7
Y1 - 2012/7
N2 - The aim of this work was to study the feasibility of detection of Trichinella spiralis in swine meat using Middle Infrared Spectroscopy Fourier Transform with Attenuated Total Reflectance (ATR) and Soft Independent Modeling of Class Analogy (MID-FTIR-ATR-SIMCA). Five male Pigs were orally infected at different larvae concentrations (13,000, 6500, 3500, 1625, 812 larvae/pig) and after 24. weeks the animals were euthanized. Five types of muscles were studied (leg, loin, rib, masseter, and diaphragm). Results showed that MID-FTIR-ATR-SIMCA was useful to determine the presence of T. spiralis in the samples, as the interclass distance between infected and non infected muscles varied from 13.5 to 36.8. This technique was also useful to discriminate among pig muscles, where masseter showed the largest interclass distance, while rib presented the smallest one. In all cases the recognition and rejection rates were 100%, which means that the methodology is capable of accurately separating T. spiralis infected from non infected swine meat.
AB - The aim of this work was to study the feasibility of detection of Trichinella spiralis in swine meat using Middle Infrared Spectroscopy Fourier Transform with Attenuated Total Reflectance (ATR) and Soft Independent Modeling of Class Analogy (MID-FTIR-ATR-SIMCA). Five male Pigs were orally infected at different larvae concentrations (13,000, 6500, 3500, 1625, 812 larvae/pig) and after 24. weeks the animals were euthanized. Five types of muscles were studied (leg, loin, rib, masseter, and diaphragm). Results showed that MID-FTIR-ATR-SIMCA was useful to determine the presence of T. spiralis in the samples, as the interclass distance between infected and non infected muscles varied from 13.5 to 36.8. This technique was also useful to discriminate among pig muscles, where masseter showed the largest interclass distance, while rib presented the smallest one. In all cases the recognition and rejection rates were 100%, which means that the methodology is capable of accurately separating T. spiralis infected from non infected swine meat.
KW - MID-FTIR
KW - Pig meat
KW - T. spiralis
UR - http://www.scopus.com/inward/record.url?scp=84859622942&partnerID=8YFLogxK
U2 - 10.1016/j.meatsci.2012.01.019
DO - 10.1016/j.meatsci.2012.01.019
M3 - Artículo
C2 - 22364689
SN - 0309-1740
VL - 91
SP - 240
EP - 246
JO - Meat Science
JF - Meat Science
IS - 3
ER -