TY - JOUR
T1 - Determination of the hydrocarbon-degrading metabolic capabilities of tropical bacterial isolates
AU - Márquez-Rocha, Facundo J.
AU - Olmos-Soto, Jorge
AU - Concepción Rosano-Hernández, Ma
AU - Muriel-García, Manuel
N1 - Funding Information:
The authors thank Fausto Arellano and Daniel Murillo for their technical assistance. The Project No. 6489 was supported by the Instituto Mexicano del Petróleo, Zona Marina del Golfo.
PY - 2005/1
Y1 - 2005/1
N2 - Of more than 20 bacteria isolated from a tropical soil using minimal medium supplemented with hydrocarbons, 11 grew well on diesel as sole carbon source, and another 11 grew in the presence of polynuclear aromatic hydrocarbons (PAHs). Ten isolates were identified phenotypically as Pseudomonas sp. and eight as Bacillus sp. Gene sequences representing the catabolic genes (alkM, todM, ndoM, and xylM) and 16S rRNA gene sequences characteristic for Pseudomonas and Bacillus were amplified by PCR, using DNA recovered from the supernatant of hydrocarbon-contaminated soil suspensions. Based on their rapid growth characteristics in the presence of hydrocarbons and the formation of PCR products for the catabolic genes alkM and ndoM six isolates were selected for biodegradation assays. After 30 days a mixed culture of two isolates achieved close to 70% hydrocarbon removal and apparent mineralization of 16% of the hydrocarbons present in the soil. Biodegradation rates varied from 275 to 387 mg hydrocarbon kg -1day -1. Several bacterial isolates obtained in this study have catabolic capabilities for the biodegradation of alkanes and aromatic hydrocarbons including PAHs.
AB - Of more than 20 bacteria isolated from a tropical soil using minimal medium supplemented with hydrocarbons, 11 grew well on diesel as sole carbon source, and another 11 grew in the presence of polynuclear aromatic hydrocarbons (PAHs). Ten isolates were identified phenotypically as Pseudomonas sp. and eight as Bacillus sp. Gene sequences representing the catabolic genes (alkM, todM, ndoM, and xylM) and 16S rRNA gene sequences characteristic for Pseudomonas and Bacillus were amplified by PCR, using DNA recovered from the supernatant of hydrocarbon-contaminated soil suspensions. Based on their rapid growth characteristics in the presence of hydrocarbons and the formation of PCR products for the catabolic genes alkM and ndoM six isolates were selected for biodegradation assays. After 30 days a mixed culture of two isolates achieved close to 70% hydrocarbon removal and apparent mineralization of 16% of the hydrocarbons present in the soil. Biodegradation rates varied from 275 to 387 mg hydrocarbon kg -1day -1. Several bacterial isolates obtained in this study have catabolic capabilities for the biodegradation of alkanes and aromatic hydrocarbons including PAHs.
KW - Catabolic genes
KW - Hydrocarbon biodegradation
KW - Metabolic capabilities
KW - Pseudomonas
UR - http://www.scopus.com/inward/record.url?scp=12344309388&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2004.05.007
DO - 10.1016/j.ibiod.2004.05.007
M3 - Artículo
AN - SCOPUS:12344309388
SN - 0964-8305
VL - 55
SP - 17
EP - 23
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
IS - 1
ER -