TY - JOUR
T1 - Determination and evolutionary significance of nucleotide sequences near to the 3′-end of 16S ribosomal RNA of mycobacteria
AU - Estrada-G, Iris C.E.
AU - Colston, M. Joseph
AU - Cox, Robert A.
N1 - Funding Information:
This investigation received financial support from the UNDP/WHO Special programme for Research Training in Tropical Disease. I.C.E.E,-G. was funded by the British Leprosy Relief Association (LEPRA) and the British Council.
PY - 1989/10/15
Y1 - 1989/10/15
N2 - The nucleotide sequence at the 3′-end of 16Sr-RNA (nucleotides 1305-1508) was determined, by the primer extension method, for Mycobacterium smegmatis, Mycobacterium tuberculosis and Mycobacterium vaccae, in addition to Mycobacterium leprae. No differences in nucleotide sequence were detected, indicating that this region of 16SrRNA is highly conserved among mycobacteria. The nucleotide sequence common to the four above-mentioned mycobacteria differs from that reported for species of other genera. For example, for helix 39 (nucleotides 1408-1491) the mycobacterial sequence has 58% similarity with the Escherichia coli sequence, 74% similarity with the Bacillus subtilis sequence and 93% similarity with the Streptomyces sequence. The observations support the assignment of M. leprae to the genus Mycobacterium.
AB - The nucleotide sequence at the 3′-end of 16Sr-RNA (nucleotides 1305-1508) was determined, by the primer extension method, for Mycobacterium smegmatis, Mycobacterium tuberculosis and Mycobacterium vaccae, in addition to Mycobacterium leprae. No differences in nucleotide sequence were detected, indicating that this region of 16SrRNA is highly conserved among mycobacteria. The nucleotide sequence common to the four above-mentioned mycobacteria differs from that reported for species of other genera. For example, for helix 39 (nucleotides 1408-1491) the mycobacterial sequence has 58% similarity with the Escherichia coli sequence, 74% similarity with the Bacillus subtilis sequence and 93% similarity with the Streptomyces sequence. The observations support the assignment of M. leprae to the genus Mycobacterium.
KW - Mycobacteria
KW - Nucleotide sequence
KW - rRNA
UR - http://www.scopus.com/inward/record.url?scp=0024446856&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.1989.tb03638.x
DO - 10.1111/j.1574-6968.1989.tb03638.x
M3 - Artículo
SN - 0378-1097
VL - 61
SP - 285
EP - 290
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
IS - 3
ER -