Detection of Salmonella enterica on silicon substrates biofunctionalized with anti-Salmonella IgG, analyzed by FTIR spectroscopy

F. J. Gómez-Montaño, A. Orduña-Díaz, M. C.G. Avelino-Flores, F. Avelino-Flores, F. Ramos-Collazo, C. Reyes-Betanzo, V. López-Gayou

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2 Scopus citations

Abstract

© 2020, Universidad Autonoma Metropolitana Iztapalapa. All rights reserved. Salmonella enterica was detected at a concentration of 500 CFU/mL. For this, three substrates were used (crystalline and amorphous silicon, hydrogenated amorphous silicon carbide) with an area of 25 mm2, functionalized by the self-assembled monolayers (SAM) technique. Characteristic functional groups of SAMs were observed (-NH2, -C=O, Si-O-Si, Amide I, Amide II) by the Fourier transform infrared spectroscopy (FTIR) technique. Anti-Salmonella IgG antibodies were generated. To determine the secondary structure of proteins, second derivative of each FTIR detection spectrum was generated, observing modifications in the zone between 1600-1700 cm-1, which corresponds to the region of proteins, specifically to structures of β- sheets and β-turns, as a result, substrates or platforms of crystalline silicon and hydrogenated amorphous silicon carbide showed band frequencies with higher intensity, being this attributed to a better sensitivity, which are proposed for applications in the development of biosensors for the monitoring of microbial quality in foods.
Original languageAmerican English
Pages (from-to)1175-1185
Number of pages11
JournalRevista Mexicana de Ingeniera Quimica
DOIs
StatePublished - 1 Sep 2020

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