Detection of miRNAs by tissue printing and dot blot hybridization

Marcelino Martínez Núñez, Stefan de Folter, Flor de Fátima Rosas-Cárdenas

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Tissue printing and dot blot are simple techniques to detect miRNA expression and localization, allowing a better understanding of the function of a miRNA. In this work, we describe a tissue printing and a dot blot hybridization protocol for miRNA detection and localization in plant tissues, which opens the possibility of analyzing spatiotemporal expression patterns of miRNAs.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages151-157
Number of pages7
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1932
ISSN (Print)1064-3745

Keywords

  • Dot blot
  • Hybridization
  • Nylon membrane
  • Tissue printing
  • miRNA detection
  • microRNAs

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