Detection of Brucella abortus by a platform functionalized with protein A and specific antibodies IgG

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Abstract

© 2019 Wiley Periodicals, Inc. Oriented immobilization of antibodies on a sensor surface is critical for enhancing both the antigen-binding capacity and the sensitivity of immunosensors. In this study, we describe a strategy to adsorb immunoglobulin G (IgG) anti-Brucella antibodies onto a silicon surface, oriented by protein A obtained from Staphylococcus aureus (SpA). X-ray photoelectron spectroscopy and atomic force microscopy were used to characterize topographically, morphologically, and chemical changes of the sensor functionalization. The activity of the biosensor was assessed by confocal microscopy, scanning electronic microscopy, and bacteria capture assays (BCA). According to the BCA, the efficiency of Brucella abortus detection with the SpA-IgG anti Brucella biosensor was three-fold higher than that of the random orientated IgG anti Brucella biosensor. The limit of detection was 1 × 106 CFU/ml. These data show that the orientation of antibodies immobilization is crucial to developing immunosensors for bacterial antigen detection as Brucella spp and improve its sensibility level. Functionalization with protein A increases Brucella detection by an antibody-coated surface. Functionalized silicon surface for Brucella detection was characterized by atomic force microscopy, X-ray photoelectron spectroscopy and confocal microscopy.
Original languageAmerican English
JournalMicroscopy Research and Technique
DOIs
StatePublished - 1 Jan 2019

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Brucella abortus
Brucella
Staphylococcal Protein A
antibodies
Antibodies
platforms
Immunoglobulin G
Biosensors
proteins
Proteins
Immunosensors
bioinstrumentation
Confocal microscopy
Biosensing Techniques
Antigens
staphylococcus
antigens
Photoelectron Spectroscopy
Assays
Atomic force microscopy

Cite this

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title = "Detection of Brucella abortus by a platform functionalized with protein A and specific antibodies IgG",
abstract = "{\circledC} 2019 Wiley Periodicals, Inc. Oriented immobilization of antibodies on a sensor surface is critical for enhancing both the antigen-binding capacity and the sensitivity of immunosensors. In this study, we describe a strategy to adsorb immunoglobulin G (IgG) anti-Brucella antibodies onto a silicon surface, oriented by protein A obtained from Staphylococcus aureus (SpA). X-ray photoelectron spectroscopy and atomic force microscopy were used to characterize topographically, morphologically, and chemical changes of the sensor functionalization. The activity of the biosensor was assessed by confocal microscopy, scanning electronic microscopy, and bacteria capture assays (BCA). According to the BCA, the efficiency of Brucella abortus detection with the SpA-IgG anti Brucella biosensor was three-fold higher than that of the random orientated IgG anti Brucella biosensor. The limit of detection was 1 × 106 CFU/ml. These data show that the orientation of antibodies immobilization is crucial to developing immunosensors for bacterial antigen detection as Brucella spp and improve its sensibility level. Functionalization with protein A increases Brucella detection by an antibody-coated surface. Functionalized silicon surface for Brucella detection was characterized by atomic force microscopy, X-ray photoelectron spectroscopy and confocal microscopy.",
author = "Baltierra-Uribe, {Shantal Lizbeth} and Chanona-P{\'e}rez, {Jos{\'e} Jorge} and M{\'e}ndez-M{\'e}ndez, {Juan Vicente} and Perea-Flores, {Mar{\'i}a de Jes{\'u}s} and S{\'a}nchez-Ch{\'a}vez, {Anah{\'i} Carolina} and Garc{\'i}a-P{\'e}rez, {Blanca Estela} and Moreno-Lafont, {Martha Cecilia} and Rub{\'e}n L{\'o}pez-Santiago",
year = "2019",
month = "1",
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doi = "10.1002/jemt.23206",
language = "American English",
journal = "Microscopy Research and Technique",
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T1 - Detection of Brucella abortus by a platform functionalized with protein A and specific antibodies IgG

AU - Baltierra-Uribe, Shantal Lizbeth

AU - Chanona-Pérez, José Jorge

AU - Méndez-Méndez, Juan Vicente

AU - Perea-Flores, María de Jesús

AU - Sánchez-Chávez, Anahí Carolina

AU - García-Pérez, Blanca Estela

AU - Moreno-Lafont, Martha Cecilia

AU - López-Santiago, Rubén

PY - 2019/1/1

Y1 - 2019/1/1

N2 - © 2019 Wiley Periodicals, Inc. Oriented immobilization of antibodies on a sensor surface is critical for enhancing both the antigen-binding capacity and the sensitivity of immunosensors. In this study, we describe a strategy to adsorb immunoglobulin G (IgG) anti-Brucella antibodies onto a silicon surface, oriented by protein A obtained from Staphylococcus aureus (SpA). X-ray photoelectron spectroscopy and atomic force microscopy were used to characterize topographically, morphologically, and chemical changes of the sensor functionalization. The activity of the biosensor was assessed by confocal microscopy, scanning electronic microscopy, and bacteria capture assays (BCA). According to the BCA, the efficiency of Brucella abortus detection with the SpA-IgG anti Brucella biosensor was three-fold higher than that of the random orientated IgG anti Brucella biosensor. The limit of detection was 1 × 106 CFU/ml. These data show that the orientation of antibodies immobilization is crucial to developing immunosensors for bacterial antigen detection as Brucella spp and improve its sensibility level. Functionalization with protein A increases Brucella detection by an antibody-coated surface. Functionalized silicon surface for Brucella detection was characterized by atomic force microscopy, X-ray photoelectron spectroscopy and confocal microscopy.

AB - © 2019 Wiley Periodicals, Inc. Oriented immobilization of antibodies on a sensor surface is critical for enhancing both the antigen-binding capacity and the sensitivity of immunosensors. In this study, we describe a strategy to adsorb immunoglobulin G (IgG) anti-Brucella antibodies onto a silicon surface, oriented by protein A obtained from Staphylococcus aureus (SpA). X-ray photoelectron spectroscopy and atomic force microscopy were used to characterize topographically, morphologically, and chemical changes of the sensor functionalization. The activity of the biosensor was assessed by confocal microscopy, scanning electronic microscopy, and bacteria capture assays (BCA). According to the BCA, the efficiency of Brucella abortus detection with the SpA-IgG anti Brucella biosensor was three-fold higher than that of the random orientated IgG anti Brucella biosensor. The limit of detection was 1 × 106 CFU/ml. These data show that the orientation of antibodies immobilization is crucial to developing immunosensors for bacterial antigen detection as Brucella spp and improve its sensibility level. Functionalization with protein A increases Brucella detection by an antibody-coated surface. Functionalized silicon surface for Brucella detection was characterized by atomic force microscopy, X-ray photoelectron spectroscopy and confocal microscopy.

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