TY - JOUR
T1 - Designed functional dispersion for insulin protection from pepsin degradation and skeletal muscle cell proliferation
T2 - In silico and in vitro study
AU - Chittepu, Veera C.S.R.
AU - Kalhotra, Poonam
AU - Gallardo-Velázquez, Tzayhri
AU - Robles-De La Torre, Raúl René
AU - Osorio-Revilla, Guillermo
N1 - Publisher Copyright:
© 2018 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2018/10/19
Y1 - 2018/10/19
N2 - Functionalized single-walled carbon nanotubes with polyethylene glycol (PEGylated SWCNTs) are a promising nanomaterial that recently has emerged as the most attractive “cargo” to deliver chemicals, peptides, DNA and RNAs into cells. Insulin therapy is a recommended therapy to treat diabetes mellitus despite its side effects. Recently, functional dispersion made up of bioactive peptides, bioactive compounds and functionalized carbon nanomaterials such as PEGylated SWCNTs have proved to possess promising applications in nanomedicine. In the present study, molecular modeling simulations are utilized to assist in designing insulin hormone-PEGylated SWCNT composites, also called functional dispersion, to achieve this experimentally, an ultrasonication tool was utilized. Enzymatic degradation assay revealed that the designed functional dispersion protects about 70% of free insulin from pepsin. In addition, sulforhodamine B (SRB) assay, the quantification of insulin and glucose levels in differentiated skeletal muscle cell supernatants, reveals that functional dispersion regulates glucose and insulin levels to promote skeletal muscle cell proliferation. These findings offer new perspectives for designed functional dispersion, as potential pharmaceutical preparations to improve insulin therapy and promote skeletal muscle cell health.
AB - Functionalized single-walled carbon nanotubes with polyethylene glycol (PEGylated SWCNTs) are a promising nanomaterial that recently has emerged as the most attractive “cargo” to deliver chemicals, peptides, DNA and RNAs into cells. Insulin therapy is a recommended therapy to treat diabetes mellitus despite its side effects. Recently, functional dispersion made up of bioactive peptides, bioactive compounds and functionalized carbon nanomaterials such as PEGylated SWCNTs have proved to possess promising applications in nanomedicine. In the present study, molecular modeling simulations are utilized to assist in designing insulin hormone-PEGylated SWCNT composites, also called functional dispersion, to achieve this experimentally, an ultrasonication tool was utilized. Enzymatic degradation assay revealed that the designed functional dispersion protects about 70% of free insulin from pepsin. In addition, sulforhodamine B (SRB) assay, the quantification of insulin and glucose levels in differentiated skeletal muscle cell supernatants, reveals that functional dispersion regulates glucose and insulin levels to promote skeletal muscle cell proliferation. These findings offer new perspectives for designed functional dispersion, as potential pharmaceutical preparations to improve insulin therapy and promote skeletal muscle cell health.
KW - Diabetes
KW - Functional dispersion
KW - Glucose metabolism
KW - Insulin therapy
KW - PEGylated SWCNTs
KW - Pharmaceutical nanotechnology
KW - Skeletal muscle cell proliferation
UR - http://www.scopus.com/inward/record.url?scp=85056315198&partnerID=8YFLogxK
U2 - 10.3390/nano8100852
DO - 10.3390/nano8100852
M3 - Artículo
C2 - 30347680
AN - SCOPUS:85056315198
SN - 2079-4991
VL - 8
JO - Nanomaterials
JF - Nanomaterials
IS - 10
M1 - 852
ER -