TY - JOUR
T1 - Decellularization of porcine esophageal tissue at three diameters and the bioscaffold modification with EETs-ECM gel
AU - Orozco-Vega, Adriana
AU - Montes-Rodríguez, Metzeri I.
AU - Luévano-Colmenero, Guadalupe H.
AU - Barros-Gómez, Jimena
AU - Muñoz-González, Pedro U.
AU - Flores-Moreno, Mauricio
AU - Delgadillo-Holtfort, Isabel
AU - Vega-González, Arturo
AU - Rojo, Francisco J.
AU - Guinea, Gustavo V.
AU - Mendoza-Novelo, Birzabith
N1 - Publisher Copyright:
© 2022 Wiley Periodicals LLC.
PY - 2022/10
Y1 - 2022/10
N2 - Damaged complex modular organs repair is a current clinical challenge in which one of the primary goals is to keep their biological response. An interesting case of study it is the porcine esophagus since it is a tubular muscular tissue selected as raw material for tissue engineering. The design of esophageal constructs can draw on properties of the processed homologous extracellular matrix (ECM). In this work, we report the decellularization of multilayered esophagus tissue from 1-, 21- and 45-days old piglets through the combination of reversible alkaline swelling and detergent perfusion. The bioscaffolds were characterized in terms of their residual composition and tensile mechanical properties. The biological response to esophageal submucosal derived bioscaffolds modified with ECM gel containing epoxyeicosatrienoic acids (EETs) was then evaluated. Results suggest that the composition (laminin, fibronectin, and sulphated glycosaminoglycans/sGAG) depends on the donor age: a better efficiency of the decellularization process combined with a higher retention of sGAG and fibronectin is observed in piglet esophageal scaffolds. The heterogeneity of this esophageal ECM is maintained, which implied the preservation of anisotropic tensile properties. Coating of bioscaffolds with ECM gel is suitable for carrying esophageal epithelial cells and EETs. Bioactivity of EETs-ECM gel modified esophageal submucosal bioscaffolds is observed to promote neovascularization and antiinflammatory after rabbit full-thickness esophageal defect replacement.
AB - Damaged complex modular organs repair is a current clinical challenge in which one of the primary goals is to keep their biological response. An interesting case of study it is the porcine esophagus since it is a tubular muscular tissue selected as raw material for tissue engineering. The design of esophageal constructs can draw on properties of the processed homologous extracellular matrix (ECM). In this work, we report the decellularization of multilayered esophagus tissue from 1-, 21- and 45-days old piglets through the combination of reversible alkaline swelling and detergent perfusion. The bioscaffolds were characterized in terms of their residual composition and tensile mechanical properties. The biological response to esophageal submucosal derived bioscaffolds modified with ECM gel containing epoxyeicosatrienoic acids (EETs) was then evaluated. Results suggest that the composition (laminin, fibronectin, and sulphated glycosaminoglycans/sGAG) depends on the donor age: a better efficiency of the decellularization process combined with a higher retention of sGAG and fibronectin is observed in piglet esophageal scaffolds. The heterogeneity of this esophageal ECM is maintained, which implied the preservation of anisotropic tensile properties. Coating of bioscaffolds with ECM gel is suitable for carrying esophageal epithelial cells and EETs. Bioactivity of EETs-ECM gel modified esophageal submucosal bioscaffolds is observed to promote neovascularization and antiinflammatory after rabbit full-thickness esophageal defect replacement.
KW - ECM composition
KW - esophagus
KW - tensile properties
KW - tissue decellularization
UR - http://www.scopus.com/inward/record.url?scp=85131813870&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.37416
DO - 10.1002/jbm.a.37416
M3 - Artículo
C2 - 35703732
AN - SCOPUS:85131813870
SN - 1549-3296
VL - 110
SP - 1669
EP - 1680
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
IS - 10
ER -