czcD gene from Bacillus megaterium and Microbacterium liquefaciens as a potential nickel–vanadium soil pollution biomarker

Grisel Fierros-Romero, Marlenne Gómez-Ramírez, Ashutosh Sharma, Reynaldo C. Pless, Norma G. Rojas-Avelizapa

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Metals are among the most prevalent pollutants released into the environment. For these reasons, the use of biomarkers for environmental monitoring of individuals and populations exposed to metal pollution has gained considerable attention, offering fast and sensitive detection of chemical stress in organisms. There are different metal resistance genes in bacteria that can be used as biomarkers, including cation diffusion facilitators carrying metal ions; the prototype is the cobalt–zinc–cadmium transporter (czcD). The present study reports the expression changes in the czcD gene in Bacillus megaterium and Microbacterium liquefaciens under nickel and vanadium exposure by real-time polymerase chain reaction. The nickel–vanadium-resistant strains of B. megaterium and M. liquefaciens used in this study were isolated from mine tailings in Guanajuato, Mexico. The czcD gene showed high expression under exposure to 200 ppm of Ni and 200 ppm of V during the logarithmic growth phase of M. liquefaciens in PHGII liquid media. In contrast, no changes were observed in B. megaterium during logarithmic and stationary growth, perhaps due to the gene having differential expression during the growth phases. The expression profiles obtained for czcD show the possibility of using this gene from M. liquefaciens as a biomarker of nickel and vanadium pollution in microorganisms.

    Original languageEnglish
    Pages (from-to)22-26
    Number of pages5
    JournalJournal of Basic Microbiology
    Volume60
    Issue number1
    DOIs
    StateAccepted/In press - 1 Jan 2019

    Keywords

    • Bacillus megaterium
    • biomarkers
    • czcD
    • Microbacterium liquefaciens
    • nickel–vanadium resistance

    Fingerprint

    Dive into the research topics of 'czcD gene from Bacillus megaterium and Microbacterium liquefaciens as a potential nickel–vanadium soil pollution biomarker'. Together they form a unique fingerprint.

    Cite this