TY - JOUR
T1 - Cytotoxic, pro-apoptotic, pro-oxidant, and non-genotoxic activities of a novel copper(II) complex against human cervical cancer
AU - Frías González, Susana E.
AU - Angeles Anguiano, Enrique
AU - Mendoza Herrera, Alberto
AU - Escutia Calzada, Daniel
AU - Ordaz Pichardo, Cynthia
N1 - Funding Information:
We thank Antonio Nieto Camacho, M. Sc. and Iván Arias González, M. Sc. for technical assistance with the TBARS and DPPH, and DNA ladder assays, respectively. This project was financially supported by the Instituto Politécnico Nacional (IPN) , Mexico (SIP Projects 20101395, 20110808, and 20121122). SEFG is student currently enrolled in the Doctoral Program of Sciences in Biotechnology at ENMH-IPN and is a recipient of student fellowships from CONACyT (40069) and PIFI-IPN.
PY - 2013/12/6
Y1 - 2013/12/6
N2 - Cisplatin remains one of the most effective current chemotherapeutic agents; however, metal complexes synthesis has increased in order to produce new anti-neoplastic drugs with DNA binding and apoptotic activities in tumor cells and less toxicity for patients. In this study, we evaluated the cytotoxic activity of a novel copper(II) complex (LQM402) against cervical cancer cell lines and found that LQM402 exhibited selective cytotoxicity against HeLa and Ca Ski cells. FITC-annexin assay and DNA fragmentation indicated that apoptosis could be involved in HeLa cell death. Caspase 3/7 and cytochrome c analysis by immunoblotting suggest the intrinsic pathway. LQM402 is a lipid peroxidation inductor according to TBARS production. Additionally, the Ames and micronucleus tests demonstrated non-genotoxic activity for this compound in Salmonella typhimurium and CD1 mice, respectively. Therefore, LQM402 may be a promising and safe anti-cervical cancer compound.
AB - Cisplatin remains one of the most effective current chemotherapeutic agents; however, metal complexes synthesis has increased in order to produce new anti-neoplastic drugs with DNA binding and apoptotic activities in tumor cells and less toxicity for patients. In this study, we evaluated the cytotoxic activity of a novel copper(II) complex (LQM402) against cervical cancer cell lines and found that LQM402 exhibited selective cytotoxicity against HeLa and Ca Ski cells. FITC-annexin assay and DNA fragmentation indicated that apoptosis could be involved in HeLa cell death. Caspase 3/7 and cytochrome c analysis by immunoblotting suggest the intrinsic pathway. LQM402 is a lipid peroxidation inductor according to TBARS production. Additionally, the Ames and micronucleus tests demonstrated non-genotoxic activity for this compound in Salmonella typhimurium and CD1 mice, respectively. Therefore, LQM402 may be a promising and safe anti-cervical cancer compound.
KW - Apoptosis
KW - Cervical cancer cells
KW - Copper(II) complex
KW - Cytotoxicity
KW - Genotoxicity
UR - http://www.scopus.com/inward/record.url?scp=84886861596&partnerID=8YFLogxK
U2 - 10.1016/j.tox.2013.08.018
DO - 10.1016/j.tox.2013.08.018
M3 - Artículo
C2 - 24012731
SN - 0300-483X
VL - 314
SP - 155
EP - 165
JO - Toxicology
JF - Toxicology
IS - 1
ER -