TY - JOUR
T1 - Cytotoxic, antioxidant and binding properties of polyphenols from the selected gluten-free pseudocereals and their by-products
T2 - In vitro model
AU - Paśko, Paweł
AU - Tyszka-Czochara, Małgorzata
AU - Namieśnik, Jacek
AU - Jastrzębski, Zenon
AU - Leontowicz, Hanna
AU - Drzewiecki, Jerzy
AU - Martinez-Ayala, Alma Leticia
AU - Nemirovski, Alina
AU - Barasch, Dinorah
AU - Gorinstein, Shela
N1 - Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/5
Y1 - 2019/5
N2 - Methanol extracts of gluten-free pseudocereals (seeds of some Amaranthus species, Chenopodium quinoa and Fagopyrum esculentum)and their by-products (flour, popping, flakes and roasted seeds)were evaluated by Folin–Ciocalteau method, mass spectrometry and fluorescence analysis in order to determine total phenolic content. The values obtained by ABTS, FRAP and CUPRAC radical scavenging assays were used to determine antioxidant capacities. The binding properties of polyphenols were evaluated by their interactions with human serum albumin (HSA). Additionally, in this study were compared selected pseudocereal seeds and by-products in terms of their cytotoxic activity against human cervical carcinoma cell lines (C4-I, HTB-35, HTB-34). The obtained results showed high phenolic contents and their antioxidant, cytotoxic and binding activities in buckwheat following by quinoa and amaranth. The investigated pseudocereals could be used as additives to increase the bioactivity and health properties in foods.
AB - Methanol extracts of gluten-free pseudocereals (seeds of some Amaranthus species, Chenopodium quinoa and Fagopyrum esculentum)and their by-products (flour, popping, flakes and roasted seeds)were evaluated by Folin–Ciocalteau method, mass spectrometry and fluorescence analysis in order to determine total phenolic content. The values obtained by ABTS, FRAP and CUPRAC radical scavenging assays were used to determine antioxidant capacities. The binding properties of polyphenols were evaluated by their interactions with human serum albumin (HSA). Additionally, in this study were compared selected pseudocereal seeds and by-products in terms of their cytotoxic activity against human cervical carcinoma cell lines (C4-I, HTB-35, HTB-34). The obtained results showed high phenolic contents and their antioxidant, cytotoxic and binding activities in buckwheat following by quinoa and amaranth. The investigated pseudocereals could be used as additives to increase the bioactivity and health properties in foods.
KW - Antioxidant activity
KW - Binding to proteins
KW - Cytotoxicity
KW - Gluten-free pseudocereals
UR - http://www.scopus.com/inward/record.url?scp=85065821278&partnerID=8YFLogxK
U2 - 10.1016/j.jcs.2019.04.009
DO - 10.1016/j.jcs.2019.04.009
M3 - Artículo
AN - SCOPUS:85065821278
SN - 0733-5210
VL - 87
SP - 325
EP - 333
JO - Journal of Cereal Science
JF - Journal of Cereal Science
ER -
