Cytotoxic, antioxidant and binding properties of polyphenols from the selected gluten-free pseudocereals and their by-products: In vitro model

Paweł Paśko, Małgorzata Tyszka-Czochara, Jacek Namieśnik, Zenon Jastrzębski, Hanna Leontowicz, Jerzy Drzewiecki, Alma Leticia Martinez-Ayala, Alina Nemirovski, Dinorah Barasch, Shela Gorinstein

Research output: Contribution to journalArticle

Abstract

Methanol extracts of gluten-free pseudocereals (seeds of some Amaranthus species, Chenopodium quinoa and Fagopyrum esculentum)and their by-products (flour, popping, flakes and roasted seeds)were evaluated by Folin–Ciocalteau method, mass spectrometry and fluorescence analysis in order to determine total phenolic content. The values obtained by ABTS, FRAP and CUPRAC radical scavenging assays were used to determine antioxidant capacities. The binding properties of polyphenols were evaluated by their interactions with human serum albumin (HSA). Additionally, in this study were compared selected pseudocereal seeds and by-products in terms of their cytotoxic activity against human cervical carcinoma cell lines (C4-I, HTB-35, HTB-34). The obtained results showed high phenolic contents and their antioxidant, cytotoxic and binding activities in buckwheat following by quinoa and amaranth. The investigated pseudocereals could be used as additives to increase the bioactivity and health properties in foods.

Original languageEnglish
Pages (from-to)325-333
Number of pages9
JournalJournal of Cereal Science
Volume87
DOIs
StatePublished - May 2019

Fingerprint

Glutens
binding properties
Polyphenols
Chenopodium quinoa
gluten
Fagopyrum
byproducts
Byproducts
Seed
Seeds
polyphenols
Antioxidants
antioxidants
seeds
popping
Amaranthus
amaranth grain
Fagopyrum esculentum
uterine cervical neoplasms
flakes

Keywords

  • Antioxidant activity
  • Binding to proteins
  • Cytotoxicity
  • Gluten-free pseudocereals

Cite this

Paśko, P., Tyszka-Czochara, M., Namieśnik, J., Jastrzębski, Z., Leontowicz, H., Drzewiecki, J., ... Gorinstein, S. (2019). Cytotoxic, antioxidant and binding properties of polyphenols from the selected gluten-free pseudocereals and their by-products: In vitro model. Journal of Cereal Science, 87, 325-333. https://doi.org/10.1016/j.jcs.2019.04.009
Paśko, Paweł ; Tyszka-Czochara, Małgorzata ; Namieśnik, Jacek ; Jastrzębski, Zenon ; Leontowicz, Hanna ; Drzewiecki, Jerzy ; Martinez-Ayala, Alma Leticia ; Nemirovski, Alina ; Barasch, Dinorah ; Gorinstein, Shela. / Cytotoxic, antioxidant and binding properties of polyphenols from the selected gluten-free pseudocereals and their by-products : In vitro model. In: Journal of Cereal Science. 2019 ; Vol. 87. pp. 325-333.
@article{02a3e4b96667447ba898e7d12fa8fc4c,
title = "Cytotoxic, antioxidant and binding properties of polyphenols from the selected gluten-free pseudocereals and their by-products: In vitro model",
abstract = "Methanol extracts of gluten-free pseudocereals (seeds of some Amaranthus species, Chenopodium quinoa and Fagopyrum esculentum)and their by-products (flour, popping, flakes and roasted seeds)were evaluated by Folin–Ciocalteau method, mass spectrometry and fluorescence analysis in order to determine total phenolic content. The values obtained by ABTS, FRAP and CUPRAC radical scavenging assays were used to determine antioxidant capacities. The binding properties of polyphenols were evaluated by their interactions with human serum albumin (HSA). Additionally, in this study were compared selected pseudocereal seeds and by-products in terms of their cytotoxic activity against human cervical carcinoma cell lines (C4-I, HTB-35, HTB-34). The obtained results showed high phenolic contents and their antioxidant, cytotoxic and binding activities in buckwheat following by quinoa and amaranth. The investigated pseudocereals could be used as additives to increase the bioactivity and health properties in foods.",
keywords = "Antioxidant activity, Binding to proteins, Cytotoxicity, Gluten-free pseudocereals",
author = "Paweł Paśko and Małgorzata Tyszka-Czochara and Jacek Namieśnik and Zenon Jastrzębski and Hanna Leontowicz and Jerzy Drzewiecki and Martinez-Ayala, {Alma Leticia} and Alina Nemirovski and Dinorah Barasch and Shela Gorinstein",
year = "2019",
month = "5",
doi = "10.1016/j.jcs.2019.04.009",
language = "Ingl{\'e}s",
volume = "87",
pages = "325--333",
journal = "Journal of Cereal Science",
issn = "0733-5210",
publisher = "Academic Press Inc.",

}

Paśko, P, Tyszka-Czochara, M, Namieśnik, J, Jastrzębski, Z, Leontowicz, H, Drzewiecki, J, Martinez-Ayala, AL, Nemirovski, A, Barasch, D & Gorinstein, S 2019, 'Cytotoxic, antioxidant and binding properties of polyphenols from the selected gluten-free pseudocereals and their by-products: In vitro model', Journal of Cereal Science, vol. 87, pp. 325-333. https://doi.org/10.1016/j.jcs.2019.04.009

Cytotoxic, antioxidant and binding properties of polyphenols from the selected gluten-free pseudocereals and their by-products : In vitro model. / Paśko, Paweł; Tyszka-Czochara, Małgorzata; Namieśnik, Jacek; Jastrzębski, Zenon; Leontowicz, Hanna; Drzewiecki, Jerzy; Martinez-Ayala, Alma Leticia; Nemirovski, Alina; Barasch, Dinorah; Gorinstein, Shela.

In: Journal of Cereal Science, Vol. 87, 05.2019, p. 325-333.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cytotoxic, antioxidant and binding properties of polyphenols from the selected gluten-free pseudocereals and their by-products

T2 - In vitro model

AU - Paśko, Paweł

AU - Tyszka-Czochara, Małgorzata

AU - Namieśnik, Jacek

AU - Jastrzębski, Zenon

AU - Leontowicz, Hanna

AU - Drzewiecki, Jerzy

AU - Martinez-Ayala, Alma Leticia

AU - Nemirovski, Alina

AU - Barasch, Dinorah

AU - Gorinstein, Shela

PY - 2019/5

Y1 - 2019/5

N2 - Methanol extracts of gluten-free pseudocereals (seeds of some Amaranthus species, Chenopodium quinoa and Fagopyrum esculentum)and their by-products (flour, popping, flakes and roasted seeds)were evaluated by Folin–Ciocalteau method, mass spectrometry and fluorescence analysis in order to determine total phenolic content. The values obtained by ABTS, FRAP and CUPRAC radical scavenging assays were used to determine antioxidant capacities. The binding properties of polyphenols were evaluated by their interactions with human serum albumin (HSA). Additionally, in this study were compared selected pseudocereal seeds and by-products in terms of their cytotoxic activity against human cervical carcinoma cell lines (C4-I, HTB-35, HTB-34). The obtained results showed high phenolic contents and their antioxidant, cytotoxic and binding activities in buckwheat following by quinoa and amaranth. The investigated pseudocereals could be used as additives to increase the bioactivity and health properties in foods.

AB - Methanol extracts of gluten-free pseudocereals (seeds of some Amaranthus species, Chenopodium quinoa and Fagopyrum esculentum)and their by-products (flour, popping, flakes and roasted seeds)were evaluated by Folin–Ciocalteau method, mass spectrometry and fluorescence analysis in order to determine total phenolic content. The values obtained by ABTS, FRAP and CUPRAC radical scavenging assays were used to determine antioxidant capacities. The binding properties of polyphenols were evaluated by their interactions with human serum albumin (HSA). Additionally, in this study were compared selected pseudocereal seeds and by-products in terms of their cytotoxic activity against human cervical carcinoma cell lines (C4-I, HTB-35, HTB-34). The obtained results showed high phenolic contents and their antioxidant, cytotoxic and binding activities in buckwheat following by quinoa and amaranth. The investigated pseudocereals could be used as additives to increase the bioactivity and health properties in foods.

KW - Antioxidant activity

KW - Binding to proteins

KW - Cytotoxicity

KW - Gluten-free pseudocereals

UR - http://www.scopus.com/inward/record.url?scp=85065821278&partnerID=8YFLogxK

U2 - 10.1016/j.jcs.2019.04.009

DO - 10.1016/j.jcs.2019.04.009

M3 - Artículo

AN - SCOPUS:85065821278

VL - 87

SP - 325

EP - 333

JO - Journal of Cereal Science

JF - Journal of Cereal Science

SN - 0733-5210

ER -