TY - JOUR
T1 - Cytotoxic and genotoxic activity of protein isolate of ayocote beans and anticancer activity of their protein fractions
AU - Teniente-Martínez, Gerardo
AU - Bernardino-Nicanor, Aurea
AU - Cariño-Cortés, Raquel
AU - Valadez-Vega, María del Carmen
AU - Montañez-Soto, José Luis
AU - Acosta-García, Gerardo
AU - González-Cruz, Leopoldo
N1 - Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2019/6/15
Y1 - 2019/6/15
N2 - We evaluated the cytotoxic and genotoxic effects of protein isolates of ayocote beans on adult male CD1 + mice and the anticancer activity of their protein fractions using SiHa Cells. The mice that received protein isolate showed a Polychromatic Erythrocytes (PCE) increase at 48 h; a significant PCE decrease was observed in mice administered the protein isolate-Daunorubicin (isolate-DAU) mix. A micronucleated erythrocytes (MNE) increase was observed in mice that received the mix in all times tested. All protein fractions of ayocote beans (black and purple) showed inhibition against SiHa cells proliferation at doses of 3000 or 5000 µg/mL. The maximal inhibitory concentration (IC 50 ) of each protein fraction was reached at a concentration of 5000 µg/mL. A synergistic effect of isolate-DAU was observed in the in vivo test; the effect of the protein fractions on SiHa cells proliferation depended of the ayocote bean variety used.
AB - We evaluated the cytotoxic and genotoxic effects of protein isolates of ayocote beans on adult male CD1 + mice and the anticancer activity of their protein fractions using SiHa Cells. The mice that received protein isolate showed a Polychromatic Erythrocytes (PCE) increase at 48 h; a significant PCE decrease was observed in mice administered the protein isolate-Daunorubicin (isolate-DAU) mix. A micronucleated erythrocytes (MNE) increase was observed in mice that received the mix in all times tested. All protein fractions of ayocote beans (black and purple) showed inhibition against SiHa cells proliferation at doses of 3000 or 5000 µg/mL. The maximal inhibitory concentration (IC 50 ) of each protein fraction was reached at a concentration of 5000 µg/mL. A synergistic effect of isolate-DAU was observed in the in vivo test; the effect of the protein fractions on SiHa cells proliferation depended of the ayocote bean variety used.
KW - Ayocote bean
KW - Cytotoxic
KW - Genotoxic
KW - Protein fractions
KW - SiHa cells
UR - http://www.scopus.com/inward/record.url?scp=85059538071&partnerID=8YFLogxK
U2 - 10.1007/s11694-018-0019-7
DO - 10.1007/s11694-018-0019-7
M3 - Artículo
SN - 2193-4126
VL - 13
SP - 1040
EP - 1048
JO - Journal of Food Measurement and Characterization
JF - Journal of Food Measurement and Characterization
IS - 2
ER -