Detección de Gen CYP4 en el Genoma de Cryptolaemus montrouzieri en Base a Secuencias Ortólogas y Uso de Codones

Translated title of the contribution: CYP4 Gene Detection in Cryptolaemus mountrouzieri Genome Based on Ortholog Sequences and Codon Usage

Merced José Eduardo Jiménez-Diosdado, Maribel Mireles-Martínez, Jesús M. Villegas-Mendoza, Raymundo Rosas-Quijano, Ninfa M. Rosas-García

Research output: Contribution to journalArticlepeer-review

Abstract

In this study we detected the CYP4 gene from Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae) by designing oligonucleotides through the selection of seven partial protein sequences of Anopheles gambiae Giles (Diptera: Culicidae) associated with the CYP4 gene, and analyzed by codon usage according to eight genes from Harmonia axyridis Pallas (Coleoptera: Coccinellidae) which is closely related to C. montrouzieri. Orthologous amino acid sequences were aligned to identify areas of homology and a table of codon usage for H. axyridis was developed. The nucleotide sequences obtained were analyzed using the program GCUA to determine the most commonly used codons, reduce degeneracy and have better amplification. Amplified products were cloned and sequenced. The effective number of codons for H. axyridis was 2996 and a predominant use of codons with AT is observed. Oligonucleotides designed 5'GAAGUUGAUACHUUUAUGUUUG3' and 3'AAARTTTTCWGGRTTTGGAWA5' amplified a 569 bp fragment with significant alignment to a partial sequence of cytochrome P450 in Aedes aegypti Linnaeus (Diptera: Culicidae) and Tribolium castaneum Herbst (Coleoptera: Tenebrionidae). Using orthologous sequences and their analysis with codon usage is useful for the molecular identification of genes of interest.

Translated title of the contributionCYP4 Gene Detection in Cryptolaemus mountrouzieri Genome Based on Ortholog Sequences and Codon Usage
Original languageSpanish
Pages (from-to)771-782
Number of pages12
JournalSouthwestern Entomologist
Volume41
Issue number3
DOIs
StatePublished - Sep 2016

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