This paper discusses the regulation of Bt-maize and transgenic insects in Mexico, pointing out the current state of the use of these biotechnological developments. In addition, as insect biotechnology is new for Mexico, regulatory protocols are still under development. Technical barriers to using Bacillus thuringensis (Bt)-maize for pest control in Mexico have been deployed by regulatory uncertainty which, associated with cultural factors, underplay biological motives. Although the use of Bt-maize is still controversial in Mexico, since 2009, Bt-maize has been cultivated experimentally. © 2010 The Authors. Journal compilation © 2010 The Entomological Society of Korea and Blackwell Publishing Asia Pty Ltd.