Culture supernatants from V. cholerae O1 ElTor strains isolated from different geographic areas induce cell vacuolation and cytotoxicity

Jorge E. Vidal, Fernando Enríquez-Rincón, Silvia Giono-Cerezo, Rosa María Ribas-Aparicio, Paula Figueroa-Arredondo

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4 Citations (Scopus)

Abstract

Objective: To investigate whether the HlyA-induced vacuolating effect is produced by V. cholerae O1 ElTor strains isolated from different geographic origins, including Mexico. Material and Methods: Supernatant-induced haemolysis, vacuolating activity and cytotoxicity in Vero cells were recorded. PCR, RFLP analysis and molecular cloning were performed. Results: All ElTor strains analyzed induced cellular vacuolation. Ribotype 2 strains isolates from the U.S. gulf coast yielded the highest titer of vacuolating activity. Eight of nine strains were haemolytic, while all strains were PCR positive for the hlyA gene. We cloned the hlyA gene from two ElTor strains, a toxigenic (2514-88, ctxAB + ) and a non-toxigenic Mexican strain (CM 91-3, ctxAB - ). Supernatant from those recombinant E. coli strains induced haemolysis, cell vacuolation and cytotoxicity. RFLP-PCR analysis revealed similarities in the hlyA gene from all strains tested. Conclusion: The HlyA-induced vacuolating effect is a widespread phenotype of epidemic V. cholerae O1 ElTor strains.
Original languageAmerican English
Pages (from-to)39-47
Number of pages9
JournalSalud Publica de Mexico
DOIs
StatePublished - 1 Jan 2009

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Cholera
Hemolysis
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction
Genes
Ribotyping
Vero Cells
Molecular Cloning
Mexico
Escherichia coli
Phenotype

Cite this

@article{68c027d7679e43c292d7b320ad7fa91f,
title = "Culture supernatants from V. cholerae O1 ElTor strains isolated from different geographic areas induce cell vacuolation and cytotoxicity",
abstract = "Objective: To investigate whether the HlyA-induced vacuolating effect is produced by V. cholerae O1 ElTor strains isolated from different geographic origins, including Mexico. Material and Methods: Supernatant-induced haemolysis, vacuolating activity and cytotoxicity in Vero cells were recorded. PCR, RFLP analysis and molecular cloning were performed. Results: All ElTor strains analyzed induced cellular vacuolation. Ribotype 2 strains isolates from the U.S. gulf coast yielded the highest titer of vacuolating activity. Eight of nine strains were haemolytic, while all strains were PCR positive for the hlyA gene. We cloned the hlyA gene from two ElTor strains, a toxigenic (2514-88, ctxAB + ) and a non-toxigenic Mexican strain (CM 91-3, ctxAB - ). Supernatant from those recombinant E. coli strains induced haemolysis, cell vacuolation and cytotoxicity. RFLP-PCR analysis revealed similarities in the hlyA gene from all strains tested. Conclusion: The HlyA-induced vacuolating effect is a widespread phenotype of epidemic V. cholerae O1 ElTor strains.",
author = "Vidal, {Jorge E.} and Fernando Enr{\'i}quez-Rinc{\'o}n and Silvia Giono-Cerezo and Ribas-Aparicio, {Rosa Mar{\'i}a} and Paula Figueroa-Arredondo",
year = "2009",
month = "1",
day = "1",
doi = "10.1590/S0036-36342009000100009",
language = "American English",
pages = "39--47",
journal = "Salud Publica de Mexico",
issn = "0036-3634",
publisher = "Instituto Nacional de Salud Publica",

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Culture supernatants from V. cholerae O1 ElTor strains isolated from different geographic areas induce cell vacuolation and cytotoxicity. / Vidal, Jorge E.; Enríquez-Rincón, Fernando; Giono-Cerezo, Silvia; Ribas-Aparicio, Rosa María; Figueroa-Arredondo, Paula.

In: Salud Publica de Mexico, 01.01.2009, p. 39-47.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Culture supernatants from V. cholerae O1 ElTor strains isolated from different geographic areas induce cell vacuolation and cytotoxicity

AU - Vidal, Jorge E.

AU - Enríquez-Rincón, Fernando

AU - Giono-Cerezo, Silvia

AU - Ribas-Aparicio, Rosa María

AU - Figueroa-Arredondo, Paula

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Objective: To investigate whether the HlyA-induced vacuolating effect is produced by V. cholerae O1 ElTor strains isolated from different geographic origins, including Mexico. Material and Methods: Supernatant-induced haemolysis, vacuolating activity and cytotoxicity in Vero cells were recorded. PCR, RFLP analysis and molecular cloning were performed. Results: All ElTor strains analyzed induced cellular vacuolation. Ribotype 2 strains isolates from the U.S. gulf coast yielded the highest titer of vacuolating activity. Eight of nine strains were haemolytic, while all strains were PCR positive for the hlyA gene. We cloned the hlyA gene from two ElTor strains, a toxigenic (2514-88, ctxAB + ) and a non-toxigenic Mexican strain (CM 91-3, ctxAB - ). Supernatant from those recombinant E. coli strains induced haemolysis, cell vacuolation and cytotoxicity. RFLP-PCR analysis revealed similarities in the hlyA gene from all strains tested. Conclusion: The HlyA-induced vacuolating effect is a widespread phenotype of epidemic V. cholerae O1 ElTor strains.

AB - Objective: To investigate whether the HlyA-induced vacuolating effect is produced by V. cholerae O1 ElTor strains isolated from different geographic origins, including Mexico. Material and Methods: Supernatant-induced haemolysis, vacuolating activity and cytotoxicity in Vero cells were recorded. PCR, RFLP analysis and molecular cloning were performed. Results: All ElTor strains analyzed induced cellular vacuolation. Ribotype 2 strains isolates from the U.S. gulf coast yielded the highest titer of vacuolating activity. Eight of nine strains were haemolytic, while all strains were PCR positive for the hlyA gene. We cloned the hlyA gene from two ElTor strains, a toxigenic (2514-88, ctxAB + ) and a non-toxigenic Mexican strain (CM 91-3, ctxAB - ). Supernatant from those recombinant E. coli strains induced haemolysis, cell vacuolation and cytotoxicity. RFLP-PCR analysis revealed similarities in the hlyA gene from all strains tested. Conclusion: The HlyA-induced vacuolating effect is a widespread phenotype of epidemic V. cholerae O1 ElTor strains.

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