TY - JOUR
T1 - Cucurbitacin i elicits the formation of actin/phospho-myosin II co-aggregates by stimulation of the RhoA/ROCK pathway and inhibition of LIM-kinase
AU - Sari-Hassoun, Meryem
AU - Clement, Marie Jeanne
AU - Hamdi, Imane
AU - Bollot, Guillaume
AU - Bauvais, Cyril
AU - Joshi, Vandana
AU - Toma, Flavio
AU - Burgo, Andrea
AU - Cailleret, Michel
AU - Rosales-Hernández, Martha Cecilia
AU - Macias Pérez, Martha Edith
AU - Chabane-Sari, Daoudi
AU - Curmi, Patrick A.
N1 - Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.
PY - 2016/2/15
Y1 - 2016/2/15
N2 - Cucurbitacins are cytotoxic triterpenoid sterols isolated from plants. One of their earliest cellular effect is the aggregation of actin associated with blockage of cell migration and division that eventually lead to apoptosis. We unravel here that cucurbitacin I actually induces the co-aggregation of actin with phospho-myosin II. This co-aggregation most probably results from the stimulation of the Rho/ROCK pathway and the direct inhibition of the LIMKinase. We further provide data that suggest that the formation of these co-aggregates is independent of a putative pro-oxidant status of cucurbitacin I. The results help to understand the impact of cucurbitacins on signal transduction and actin dynamics and open novel perspectives to use it as drug candidates for cancer research.
AB - Cucurbitacins are cytotoxic triterpenoid sterols isolated from plants. One of their earliest cellular effect is the aggregation of actin associated with blockage of cell migration and division that eventually lead to apoptosis. We unravel here that cucurbitacin I actually induces the co-aggregation of actin with phospho-myosin II. This co-aggregation most probably results from the stimulation of the Rho/ROCK pathway and the direct inhibition of the LIMKinase. We further provide data that suggest that the formation of these co-aggregates is independent of a putative pro-oxidant status of cucurbitacin I. The results help to understand the impact of cucurbitacins on signal transduction and actin dynamics and open novel perspectives to use it as drug candidates for cancer research.
KW - Actin
KW - Cucurbitacin
KW - LIMK
KW - Myosin II
KW - ROCK
UR - http://www.scopus.com/inward/record.url?scp=84958780553&partnerID=8YFLogxK
U2 - 10.1016/j.bcp.2015.12.013
DO - 10.1016/j.bcp.2015.12.013
M3 - Artículo
C2 - 26707799
SN - 0006-2952
VL - 102
SP - 45
EP - 63
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
ER -