TY - JOUR
T1 - Crude Enzymatic Extract from Trametes maxima and Paecilomyces carneus Mixed Culture Entrapped on Alginate for Phenanthrene Removal in Water
AU - González Ramírez, Denisse Fabiola
AU - Rodríguez Vázquez, Refugio
AU - Avila Pérez, Pedro
AU - Torres Bustillos, Luis Gilberto
AU - López, Ricardo Aguilar
AU - Montes Horcasitas, María Del Carmen
AU - Esparza García, Fernando José
N1 - Funding Information:
The authors thank the National Institute for Nuclear Research (ININ), CINVESTAV-IPN, and CONACYT (Grant No. 355106) for the support provided through the development of this research. The study was supported by Consejo Nacional de Ciencia y Tecnología (Grant No. 355106).
PY - 2018/10
Y1 - 2018/10
N2 - The application of a crude enzymatic extract from the mixed culture of Trametes maxima (T. maxima) and Paecilomyces carneus (P. carneus) in removal of phenanthrene (PHE) from water was assessed. Production of crude extract from a mixed culture of T. maxima and P. carneus does not require special conditions yet the use of 20 g/L of glycerol as carbon source enhances laccase and manganese-dependent peroxidase activities. Application of crude extracts became feasible since it does not require further purification. The crude enzymes entrapped in alginate beads in a fixed-bed packed column reached 94.3%-0.7% of PHE removal after 13 hr of continuous treatment under nonsterile conditions, while the alginate beads without enzymes reached only 45.3%-23% of PHE removal. The difference between the removal of alginate beads with and without enzymes was the oxidative activity on the surface of the alginate beads, which was observed in the increase in the C=O group and in the X-ray spectroscopy analyses of the C 1s and O 1s regions. Beads without enzymes had less C=O groups. Polyoxyethylene sorbitan monooleate and dimethyl sulfoxide influenced enzymatic activity to carry out a level of PHE removal similar to a photocatalytic treatment.
AB - The application of a crude enzymatic extract from the mixed culture of Trametes maxima (T. maxima) and Paecilomyces carneus (P. carneus) in removal of phenanthrene (PHE) from water was assessed. Production of crude extract from a mixed culture of T. maxima and P. carneus does not require special conditions yet the use of 20 g/L of glycerol as carbon source enhances laccase and manganese-dependent peroxidase activities. Application of crude extracts became feasible since it does not require further purification. The crude enzymes entrapped in alginate beads in a fixed-bed packed column reached 94.3%-0.7% of PHE removal after 13 hr of continuous treatment under nonsterile conditions, while the alginate beads without enzymes reached only 45.3%-23% of PHE removal. The difference between the removal of alginate beads with and without enzymes was the oxidative activity on the surface of the alginate beads, which was observed in the increase in the C=O group and in the X-ray spectroscopy analyses of the C 1s and O 1s regions. Beads without enzymes had less C=O groups. Polyoxyethylene sorbitan monooleate and dimethyl sulfoxide influenced enzymatic activity to carry out a level of PHE removal similar to a photocatalytic treatment.
KW - cosolvent
KW - crude enzymatic extract
KW - entrapment
KW - hydrocarbon
KW - surfactant
UR - http://www.scopus.com/inward/record.url?scp=85055335165&partnerID=8YFLogxK
U2 - 10.1089/ees.2018.0064
DO - 10.1089/ees.2018.0064
M3 - Artículo
SN - 1092-8758
VL - 35
SP - 1126
EP - 1135
JO - Environmental Engineering Science
JF - Environmental Engineering Science
IS - 10
ER -