Construct and expression of recombinant domains I/II of dengue virus- 2 and its efficacy to evaluate immune response in endemic area: Possible use in prognosis

Alfredo Eduardo Montes-Gómez, Hector Vivanco-Cid, José Bustos-Arriaga, Mussaret Bano Zaidi, Jazmin Garcia-Machorro, Benito Gutierrez-Castañeda, Leticia Cedillo-Barron

Research output: Contribution to journalArticle

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Abstract

© 2017 Elsevier B.V. The envelope (E) protein from DENV, contain three functional and structural domains (DI, DII and DIII). Some studies suggest that neutralizing antibodies during natural DENV infection are predominantly against DI and DII, in contrast, low proportion of the antibodies were against DIII. Thus it is necessary to establish the proportion of human antibodies against DENV E protein that bind to DI and DII during the normal course of infection; as an indicator of the quality of the antibody response and to further design new vaccine candidates for DENV. The aim of this study was to express recombinant proteins harboring a 240-aminoacid fragment of the E protein from DI and DII of DENV serotypes 2 and 3 in a eukaryotic S2 system. Further, we evaluate the antibodies against these antigens in samples from patients in acute phase of DF or DHF and compare it with the response of samples from healthy individuals from the same endemic areas and samples from healthy individuals from a non-endemic area (EA and NEA, respectively). These results suggest that the presence of antibodies against rEDI/DII might be used to identify patients at risk for severe disease.
Original languageAmerican English
Pages (from-to)233-238
Number of pages209
JournalActa Tropica
DOIs
StatePublished - 1 Jul 2017

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Dengue virus
Dengue Virus
prognosis
immune response
antibodies
Antibodies
Proteins
Infection
Neutralizing Antibodies
Recombinant Proteins
proteins
Antibody Formation
neutralizing antibodies
Vaccines
recombinant proteins
sampling
infection
serotypes
Antigens
vaccines

Cite this

Montes-Gómez, Alfredo Eduardo ; Vivanco-Cid, Hector ; Bustos-Arriaga, José ; Zaidi, Mussaret Bano ; Garcia-Machorro, Jazmin ; Gutierrez-Castañeda, Benito ; Cedillo-Barron, Leticia. / Construct and expression of recombinant domains I/II of dengue virus- 2 and its efficacy to evaluate immune response in endemic area: Possible use in prognosis. In: Acta Tropica. 2017 ; pp. 233-238.
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Construct and expression of recombinant domains I/II of dengue virus- 2 and its efficacy to evaluate immune response in endemic area: Possible use in prognosis. / Montes-Gómez, Alfredo Eduardo; Vivanco-Cid, Hector; Bustos-Arriaga, José; Zaidi, Mussaret Bano; Garcia-Machorro, Jazmin; Gutierrez-Castañeda, Benito; Cedillo-Barron, Leticia.

In: Acta Tropica, 01.07.2017, p. 233-238.

Research output: Contribution to journalArticle

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AU - Zaidi, Mussaret Bano

AU - Garcia-Machorro, Jazmin

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