Conjugation of manganese ferrite nanoparticles to an anti Sticholysin monoclonal antibody and conjugate applications

V. Figueroa-Espí, A. Alvarez-Paneque, M. Torrens, A. J. Otero-González, E. Reguera

Research output: Contribution to journalArticle

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Abstract

In this study the potential applications of manganese ferrite (MnFe 2 O 4 ) nanoparticles for bioanalytical applications are evaluated. These magnetic nanoparticles show peroxidase-like activity similar to that reported for magnetite nanoparticles and peroxidase enzyme. Based on this finding, colloidal suspensions of manganese ferrite were conjugated to an anti-Sticholysin II (StII) monoclonal antibody. The resulting conjugate was then used as a revealing tool in a novel immunoassay for StII detection. From the combined magnetic properties and specific recognition of anti-StII-MnFe 2 O 4 conjugate, Sticholysins were separated from whole aqueous extract of marine anemone obtaining 75% of purity. The results herein discussed illustrate the potential applications of manganese ferrite nanoparticles as bioanalytical tools for immunoassay and protocol for protein separation. © 2011 Elsevier B.V.
Original languageAmerican English
Pages (from-to)118-124
Number of pages7
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
DOIs
StatePublished - 20 Aug 2011
Externally publishedYes

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