In this study the potential applications of manganese ferrite (MnFe 2 O 4 ) nanoparticles for bioanalytical applications are evaluated. These magnetic nanoparticles show peroxidase-like activity similar to that reported for magnetite nanoparticles and peroxidase enzyme. Based on this finding, colloidal suspensions of manganese ferrite were conjugated to an anti-Sticholysin II (StII) monoclonal antibody. The resulting conjugate was then used as a revealing tool in a novel immunoassay for StII detection. From the combined magnetic properties and specific recognition of anti-StII-MnFe 2 O 4 conjugate, Sticholysins were separated from whole aqueous extract of marine anemone obtaining 75% of purity. The results herein discussed illustrate the potential applications of manganese ferrite nanoparticles as bioanalytical tools for immunoassay and protocol for protein separation. © 2011 Elsevier B.V.
|Original language||American English|
|Number of pages||7|
|Journal||Colloids and Surfaces A: Physicochemical and Engineering Aspects|
|State||Published - 20 Aug 2011|
Figueroa-Espí, V., Alvarez-Paneque, A., Torrens, M., Otero-González, A. J., & Reguera, E. (2011). Conjugation of manganese ferrite nanoparticles to an anti Sticholysin monoclonal antibody and conjugate applications. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 118-124. https://doi.org/10.1016/j.colsurfa.2011.08.008