TY - JOUR
T1 - Conjugation of manganese ferrite nanoparticles to an anti Sticholysin monoclonal antibody and conjugate applications
AU - Figueroa-Espí, V.
AU - Alvarez-Paneque, A.
AU - Torrens, M.
AU - Otero-González, A. J.
AU - Reguera, E.
N1 - Funding Information:
This study was partially supported by the project CONACyT , 2009-01-123480 .
PY - 2011/8/20
Y1 - 2011/8/20
N2 - In this study the potential applications of manganese ferrite (MnFe2O4) nanoparticles for bioanalytical applications are evaluated. These magnetic nanoparticles show peroxidase-like activity similar to that reported for magnetite nanoparticles and peroxidase enzyme. Based on this finding, colloidal suspensions of manganese ferrite were conjugated to an anti-Sticholysin II (StII) monoclonal antibody. The resulting conjugate was then used as a revealing tool in a novel immunoassay for StII detection. From the combined magnetic properties and specific recognition of anti-StII-MnFe2O4 conjugate, Sticholysins were separated from whole aqueous extract of marine anemone obtaining 75% of purity. The results herein discussed illustrate the potential applications of manganese ferrite nanoparticles as bioanalytical tools for immunoassay and protocol for protein separation.
AB - In this study the potential applications of manganese ferrite (MnFe2O4) nanoparticles for bioanalytical applications are evaluated. These magnetic nanoparticles show peroxidase-like activity similar to that reported for magnetite nanoparticles and peroxidase enzyme. Based on this finding, colloidal suspensions of manganese ferrite were conjugated to an anti-Sticholysin II (StII) monoclonal antibody. The resulting conjugate was then used as a revealing tool in a novel immunoassay for StII detection. From the combined magnetic properties and specific recognition of anti-StII-MnFe2O4 conjugate, Sticholysins were separated from whole aqueous extract of marine anemone obtaining 75% of purity. The results herein discussed illustrate the potential applications of manganese ferrite nanoparticles as bioanalytical tools for immunoassay and protocol for protein separation.
KW - Nanoparticles bioconjugate
KW - Nanoparticles immunoassay
KW - Protein separation
KW - Sticholysin
UR - http://www.scopus.com/inward/record.url?scp=80052141698&partnerID=8YFLogxK
U2 - 10.1016/j.colsurfa.2011.08.008
DO - 10.1016/j.colsurfa.2011.08.008
M3 - Artículo
SN - 0927-7757
VL - 387
SP - 118
EP - 124
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
IS - 1-3
ER -