TY - JOUR
T1 - Complete genome sequences, before and after mammalian cell culture, of Zika virus isolated from the serum of a symptomatic male patient from Oaxaca, Mexico
AU - Boukadida, Celia
AU - Torres-Flores, Jesús M.
AU - Yocupicio-Monroy, Martha
AU - Piten-Isidro, Elvira
AU - Rivero-Arrieta, Amaranta Y.
AU - Luna-Villalobos, Yara A.
AU - Martínez-Vargas, Liliane
AU - Alcaraz-Estrada, Sofía L.
AU - Torres, Klintsy J.
AU - Lira, Rosalia
AU - Reyes-Terán, Gustavo
AU - Sevilla-Reyes, Edgar E.
N1 - Publisher Copyright:
© 2017 Boukadida et al.
PY - 2017
Y1 - 2017
N2 - Zika virus (ZIKV) is an emerging arthropod-borne flavivirus associated with severe congenital malformations and neurological complications. Although the ZIKV genome is well characterized, there is limited information regarding changes after cell isolation and culture adaptation. We isolated, and passaged in Vero cells, ZIKV from the serum of a symptomatic male patient and compared the viral genomes before and after culture. Single nucleotide polymorphisms were characteristic among serum-circulating genomes, while such diversity decreased after cell culture.
AB - Zika virus (ZIKV) is an emerging arthropod-borne flavivirus associated with severe congenital malformations and neurological complications. Although the ZIKV genome is well characterized, there is limited information regarding changes after cell isolation and culture adaptation. We isolated, and passaged in Vero cells, ZIKV from the serum of a symptomatic male patient and compared the viral genomes before and after culture. Single nucleotide polymorphisms were characteristic among serum-circulating genomes, while such diversity decreased after cell culture.
UR - http://www.scopus.com/inward/record.url?scp=85020285914&partnerID=8YFLogxK
U2 - 10.1128/genomeA.00072-17
DO - 10.1128/genomeA.00072-17
M3 - Artículo
C2 - 28336600
SN - 2169-8287
VL - 5
JO - Genome Announcements
JF - Genome Announcements
IS - 12
M1 - e00072-17
ER -