TY - JOUR
T1 - Complete genome sequence of a non-pathogenic strain of Fowl Adenovirus serotype 11
T2 - Minimal genomic differences between pathogenic and non-pathogenic viruses
AU - Absalón, Angel E.
AU - Morales-Garzón, Andrés
AU - Vera-Hernández, Pedro F.
AU - Cortés-Espinosa, Diana V.
AU - Uribe-Ochoa, Sara M.
AU - García, Laura J.
AU - Lucio-Decanini, Eduardo
N1 - Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2017/1/15
Y1 - 2017/1/15
N2 - In this study, we conducted the clinicopathological characterization of a non-pathogenic FAdV-D serotype 11 strain MX95, isolated from healthy chickens, and its entire genome was sequenced. Experiments in SPF chickens revealed that the strain is a non-pathogenic virus that did not cause death at challenge doses of 1×106 TCID50. Additionally, the infection in SPF chickens caused no apparent damage in most of the organs analyzed by necropsy and histopathology, but it did cause inclusion body hepatitis; nevertheless it did not generate severe infectious clinical symptoms. The virus was detected in several chicken organs, including the lymphoid organs, by real-time polymerase chain reaction (PCR) until 42 days. The genome of FAdV-11 MX95 has a size of 44,326 bp, and it encodes 36 open reading frames (ORFs). Comparative analysis of the genome indicated only 0.8% dissimilarity with a highly virulent serotype 11 that was previously reported.
AB - In this study, we conducted the clinicopathological characterization of a non-pathogenic FAdV-D serotype 11 strain MX95, isolated from healthy chickens, and its entire genome was sequenced. Experiments in SPF chickens revealed that the strain is a non-pathogenic virus that did not cause death at challenge doses of 1×106 TCID50. Additionally, the infection in SPF chickens caused no apparent damage in most of the organs analyzed by necropsy and histopathology, but it did cause inclusion body hepatitis; nevertheless it did not generate severe infectious clinical symptoms. The virus was detected in several chicken organs, including the lymphoid organs, by real-time polymerase chain reaction (PCR) until 42 days. The genome of FAdV-11 MX95 has a size of 44,326 bp, and it encodes 36 open reading frames (ORFs). Comparative analysis of the genome indicated only 0.8% dissimilarity with a highly virulent serotype 11 that was previously reported.
KW - FAdV-11
KW - Fowl Adenovirus
KW - Hepatitis-hydropericardium syndrome
KW - Inclusion bodies hepatitis
KW - Low pathogenic Adenovirus
UR - http://www.scopus.com/inward/record.url?scp=84995754661&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2016.11.006
DO - 10.1016/j.virol.2016.11.006
M3 - Artículo
C2 - 27865971
SN - 0042-6822
VL - 501
SP - 63
EP - 69
JO - Virology
JF - Virology
ER -