Complete genome sequence of a non-pathogenic strain of Fowl Adenovirus serotype 11: Minimal genomic differences between pathogenic and non-pathogenic viruses

Angel E. Absalón, Andrés Morales-Garzón, Pedro F. Vera-Hernández, Diana V. Cortés-Espinosa, Sara M. Uribe-Ochoa, Laura J. García, Eduardo Lucio-Decanini

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

In this study, we conducted the clinicopathological characterization of a non-pathogenic FAdV-D serotype 11 strain MX95, isolated from healthy chickens, and its entire genome was sequenced. Experiments in SPF chickens revealed that the strain is a non-pathogenic virus that did not cause death at challenge doses of 1×106 TCID50. Additionally, the infection in SPF chickens caused no apparent damage in most of the organs analyzed by necropsy and histopathology, but it did cause inclusion body hepatitis; nevertheless it did not generate severe infectious clinical symptoms. The virus was detected in several chicken organs, including the lymphoid organs, by real-time polymerase chain reaction (PCR) until 42 days. The genome of FAdV-11 MX95 has a size of 44,326 bp, and it encodes 36 open reading frames (ORFs). Comparative analysis of the genome indicated only 0.8% dissimilarity with a highly virulent serotype 11 that was previously reported.

Original languageEnglish
Pages (from-to)63-69
Number of pages7
JournalVirology
Volume501
DOIs
StatePublished - 15 Jan 2017

Keywords

  • FAdV-11
  • Fowl Adenovirus
  • Hepatitis-hydropericardium syndrome
  • Inclusion bodies hepatitis
  • Low pathogenic Adenovirus

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