TY - JOUR
T1 - Competition/antagonism associations of biofilm formation among Staphylococcus epidermidis Agr groups I, II, and III
AU - Martínez-García, Sergio
AU - Ortiz-García, César I.
AU - Cruz-Aguilar, Marisa
AU - Zenteno, Juan Carlos
AU - Murrieta-Coxca, José Martin
AU - Pérez-Tapia, Sonia Mayra
AU - Rodríguez-Martínez, Sandra
AU - Cancino-Diaz, Mario E.
AU - Cancino-Diaz, Juan C.
N1 - Publisher Copyright:
© 2019, The Microbiological Society of Korea and Springer Nature B.V.
PY - 2019/2/1
Y1 - 2019/2/1
N2 - Staphylococci have quorum-sensing (QS) systems that enable cell-to-cell communication, as well as the regulation of numerous colonization and virulence factors. The accessory gene regulator (Agr) operon is one of the Staphylococcus genus QS systems. Three groups (I, II, and III) are present in Staphylococcus epidermidis Agr operon. To date, it is unknown whether Agr groups can interact symbiotically during biofilm development. This study analyzed a symbiotic association among Agr groups during biofilm formation in clinical and commensal isolates. Different combinations among Agr group isolates was used to study biofilm formation in vitro and in vivo (using a mouse catheter-infection model). The analysis of Agr groups were also performed from samples of human skin (head, armpits, and nostrils). Different predominant coexistence was found within biofilms, suggesting symbiosis type. In vitro, Agr I had a competition with Agr II and Agr III. Agr II had a competition with Agr III, and Agr II was an antagonist to Agr I and III when the three strains were combined. In vivo, Agr II had a competition to Agr I, but Agr I and II were antagonists to Agr III. The associations found in vitro and in vivo were also found in different sites of the skin. Besides, other associations were observed: Agr III antagonized Agr I and II, and Agr III competed with Agr I and Agr II. These results suggest that, in S. epidermidis, a symbiotic association of competition and antagonism occurs among different Agr groups during biofilm formation.
AB - Staphylococci have quorum-sensing (QS) systems that enable cell-to-cell communication, as well as the regulation of numerous colonization and virulence factors. The accessory gene regulator (Agr) operon is one of the Staphylococcus genus QS systems. Three groups (I, II, and III) are present in Staphylococcus epidermidis Agr operon. To date, it is unknown whether Agr groups can interact symbiotically during biofilm development. This study analyzed a symbiotic association among Agr groups during biofilm formation in clinical and commensal isolates. Different combinations among Agr group isolates was used to study biofilm formation in vitro and in vivo (using a mouse catheter-infection model). The analysis of Agr groups were also performed from samples of human skin (head, armpits, and nostrils). Different predominant coexistence was found within biofilms, suggesting symbiosis type. In vitro, Agr I had a competition with Agr II and Agr III. Agr II had a competition with Agr III, and Agr II was an antagonist to Agr I and III when the three strains were combined. In vivo, Agr II had a competition to Agr I, but Agr I and II were antagonists to Agr III. The associations found in vitro and in vivo were also found in different sites of the skin. Besides, other associations were observed: Agr III antagonized Agr I and II, and Agr III competed with Agr I and Agr II. These results suggest that, in S. epidermidis, a symbiotic association of competition and antagonism occurs among different Agr groups during biofilm formation.
KW - Agr
KW - Staphylococcus epidermidis
KW - clinical
KW - commensal
KW - symbiosis
UR - http://www.scopus.com/inward/record.url?scp=85060830886&partnerID=8YFLogxK
U2 - 10.1007/s12275-019-8322-5
DO - 10.1007/s12275-019-8322-5
M3 - Artículo
C2 - 30706343
SN - 1225-8873
VL - 57
SP - 143
EP - 153
JO - Journal of Microbiology
JF - Journal of Microbiology
IS - 2
ER -