Comparative studies of cutins from lime (Citrus aurantifolia) and grapefruit (Citrus paradisi) after TFA hydrolysis

Brenda Liliana Hernández Velasco, Daniel Arrieta-Baez, Pedro Iván Cortez Sotelo, Juan Vicente Méndez-Méndez, Blanca Margarita Berdeja Martínez, Mayra Beatriz Gómez-Patiño

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Abstract

© 2017 Elsevier Ltd Grapefruit and lime cutins were analyzed and compared in order to obtain information about their cutin architecture. This was performed using a sequential hydrolysis, first with trifluoroacetic acid to remove most of the polysaccharides present in the cutins, followed by an alkaline hydrolysis in order to obtain the main aliphatic compounds. Analysis by CPMAS 13C NMR and ATR FT-IR of the cutins after 2.0 M TFA revealed that grapefruit cutin has independent aliphatic and polysaccharide domains while in the lime cutin these components could be homogeneously distributed. These observations were in agreement with an AFM analysis of the cutins obtained in the hydrolysis reactions. The main aliphatic compounds were detected and characterized as 16-hydroxy-10-oxo-hexadecanoic acid and 10,16-dihydroxyhexadecanoic acid. These were present in grapefruit cutin at 35.80% and 21.86% and in lime cutin at 20.44% and 40.36% respectively.
Original languageAmerican English
Pages (from-to)78-86
Number of pages69
JournalPhytochemistry
DOIs
StatePublished - 1 Dec 2017

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Citrus aurantiifolia
Citrus paradisi
cutin
Citrus
grapefruits
Lime
lime
hydrolysis
Hydrolysis
comparative study
Polysaccharides
polysaccharide
acid
Trifluoroacetic acid
Acids
nuclear magnetic resonance
Nuclear magnetic resonance
polysaccharides
Keto Acids
Trifluoroacetic Acid

Cite this

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title = "Comparative studies of cutins from lime (Citrus aurantifolia) and grapefruit (Citrus paradisi) after TFA hydrolysis",
abstract = "{\circledC} 2017 Elsevier Ltd Grapefruit and lime cutins were analyzed and compared in order to obtain information about their cutin architecture. This was performed using a sequential hydrolysis, first with trifluoroacetic acid to remove most of the polysaccharides present in the cutins, followed by an alkaline hydrolysis in order to obtain the main aliphatic compounds. Analysis by CPMAS 13C NMR and ATR FT-IR of the cutins after 2.0 M TFA revealed that grapefruit cutin has independent aliphatic and polysaccharide domains while in the lime cutin these components could be homogeneously distributed. These observations were in agreement with an AFM analysis of the cutins obtained in the hydrolysis reactions. The main aliphatic compounds were detected and characterized as 16-hydroxy-10-oxo-hexadecanoic acid and 10,16-dihydroxyhexadecanoic acid. These were present in grapefruit cutin at 35.80{\%} and 21.86{\%} and in lime cutin at 20.44{\%} and 40.36{\%} respectively.",
author = "{Hern{\'a}ndez Velasco}, {Brenda Liliana} and Daniel Arrieta-Baez and {Cortez Sotelo}, {Pedro Iv{\'a}n} and M{\'e}ndez-M{\'e}ndez, {Juan Vicente} and {Berdeja Mart{\'i}nez}, {Blanca Margarita} and G{\'o}mez-Pati{\~n}o, {Mayra Beatriz}",
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doi = "10.1016/j.phytochem.2017.08.017",
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journal = "Phytochemistry",
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Comparative studies of cutins from lime (Citrus aurantifolia) and grapefruit (Citrus paradisi) after TFA hydrolysis. / Hernández Velasco, Brenda Liliana; Arrieta-Baez, Daniel; Cortez Sotelo, Pedro Iván; Méndez-Méndez, Juan Vicente; Berdeja Martínez, Blanca Margarita; Gómez-Patiño, Mayra Beatriz.

In: Phytochemistry, 01.12.2017, p. 78-86.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Comparative studies of cutins from lime (Citrus aurantifolia) and grapefruit (Citrus paradisi) after TFA hydrolysis

AU - Hernández Velasco, Brenda Liliana

AU - Arrieta-Baez, Daniel

AU - Cortez Sotelo, Pedro Iván

AU - Méndez-Méndez, Juan Vicente

AU - Berdeja Martínez, Blanca Margarita

AU - Gómez-Patiño, Mayra Beatriz

PY - 2017/12/1

Y1 - 2017/12/1

N2 - © 2017 Elsevier Ltd Grapefruit and lime cutins were analyzed and compared in order to obtain information about their cutin architecture. This was performed using a sequential hydrolysis, first with trifluoroacetic acid to remove most of the polysaccharides present in the cutins, followed by an alkaline hydrolysis in order to obtain the main aliphatic compounds. Analysis by CPMAS 13C NMR and ATR FT-IR of the cutins after 2.0 M TFA revealed that grapefruit cutin has independent aliphatic and polysaccharide domains while in the lime cutin these components could be homogeneously distributed. These observations were in agreement with an AFM analysis of the cutins obtained in the hydrolysis reactions. The main aliphatic compounds were detected and characterized as 16-hydroxy-10-oxo-hexadecanoic acid and 10,16-dihydroxyhexadecanoic acid. These were present in grapefruit cutin at 35.80% and 21.86% and in lime cutin at 20.44% and 40.36% respectively.

AB - © 2017 Elsevier Ltd Grapefruit and lime cutins were analyzed and compared in order to obtain information about their cutin architecture. This was performed using a sequential hydrolysis, first with trifluoroacetic acid to remove most of the polysaccharides present in the cutins, followed by an alkaline hydrolysis in order to obtain the main aliphatic compounds. Analysis by CPMAS 13C NMR and ATR FT-IR of the cutins after 2.0 M TFA revealed that grapefruit cutin has independent aliphatic and polysaccharide domains while in the lime cutin these components could be homogeneously distributed. These observations were in agreement with an AFM analysis of the cutins obtained in the hydrolysis reactions. The main aliphatic compounds were detected and characterized as 16-hydroxy-10-oxo-hexadecanoic acid and 10,16-dihydroxyhexadecanoic acid. These were present in grapefruit cutin at 35.80% and 21.86% and in lime cutin at 20.44% and 40.36% respectively.

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