TY - JOUR
T1 - Comparative studies of cutins from lime (Citrus aurantifolia) and grapefruit (Citrus paradisi) after TFA hydrolysis
AU - Hernández Velasco, Brenda Liliana
AU - Arrieta-Baez, Daniel
AU - Cortez Sotelo, Pedro Iván
AU - Méndez-Méndez, Juan Vicente
AU - Berdeja Martínez, Blanca Margarita
AU - Gómez-Patiño, Mayra Beatriz
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/12
Y1 - 2017/12
N2 - Grapefruit and lime cutins were analyzed and compared in order to obtain information about their cutin architecture. This was performed using a sequential hydrolysis, first with trifluoroacetic acid to remove most of the polysaccharides present in the cutins, followed by an alkaline hydrolysis in order to obtain the main aliphatic compounds. Analysis by CPMAS 13C NMR and ATR FT-IR of the cutins after 2.0 M TFA revealed that grapefruit cutin has independent aliphatic and polysaccharide domains while in the lime cutin these components could be homogeneously distributed. These observations were in agreement with an AFM analysis of the cutins obtained in the hydrolysis reactions. The main aliphatic compounds were detected and characterized as 16-hydroxy-10-oxo-hexadecanoic acid and 10,16-dihydroxyhexadecanoic acid. These were present in grapefruit cutin at 35.80% and 21.86% and in lime cutin at 20.44% and 40.36% respectively.
AB - Grapefruit and lime cutins were analyzed and compared in order to obtain information about their cutin architecture. This was performed using a sequential hydrolysis, first with trifluoroacetic acid to remove most of the polysaccharides present in the cutins, followed by an alkaline hydrolysis in order to obtain the main aliphatic compounds. Analysis by CPMAS 13C NMR and ATR FT-IR of the cutins after 2.0 M TFA revealed that grapefruit cutin has independent aliphatic and polysaccharide domains while in the lime cutin these components could be homogeneously distributed. These observations were in agreement with an AFM analysis of the cutins obtained in the hydrolysis reactions. The main aliphatic compounds were detected and characterized as 16-hydroxy-10-oxo-hexadecanoic acid and 10,16-dihydroxyhexadecanoic acid. These were present in grapefruit cutin at 35.80% and 21.86% and in lime cutin at 20.44% and 40.36% respectively.
KW - Cuticles
KW - Cutin
KW - Grapefruit
KW - Lime
KW - Solid-state NMR
UR - http://www.scopus.com/inward/record.url?scp=85028931496&partnerID=8YFLogxK
U2 - 10.1016/j.phytochem.2017.08.017
DO - 10.1016/j.phytochem.2017.08.017
M3 - Artículo
C2 - 28898741
SN - 0031-9422
VL - 144
SP - 78
EP - 86
JO - Phytochemistry
JF - Phytochemistry
ER -