TY - JOUR
T1 - Comparative proteomic analysis of outer membrane vesicles from Brucella suis, Brucella ovis, Brucella canis and Brucella neotomae
AU - Socorro Ruiz-Palma, María del
AU - Avila-Calderón, Eric Daniel
AU - Aguilera-Arreola, Ma Guadalupe
AU - López-Merino, Ahidé
AU - Ruiz, Enrico A.
AU - Morales-García, María del Rosario
AU - López-Villegas, Edgar Oliver
AU - Gomez-Lunar, Zulema
AU - Arellano-Reynoso, Beatriz
AU - Contreras-Rodríguez, Araceli
N1 - Funding Information:
This work was supported by CONACYT CB-2011-01 No. 169259, SIP-IPN 20200594, SAGARPA-CONACYT 2017-02-291311, ICYT-DF/IPN, and CONACYT 61529. MGAA, EARC and ACR were supported by fellowships from COFAA-IPN, SIP-EDI, and SNI-CONACYT, respectively. MSRP was supported by a PRODEP and COMECYT scholarship. We thank Dr. Norma Velazquez-Guadarrama for technical support.
Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021
Y1 - 2021
N2 - Gram-negative bacteria release nanovesicles, called outer membrane vesicles (OMVs), from their outer membrane. Proteomics has been used to determine their composition. OMVs contain proteins able to elicit an immune response, so they have been proposed as a model to develop acellular vaccines. In this study, OMVs of Brucella suis, B. ovis, B. canis, and B. neotomae were purified and analyzed by SDS-PAGE, transmission electron microscopy and liquid chromatography coupled to mass spectrometry to determine the pan-proteome of these vesicles. In addition, antigenic proteins were detected by western blot with anti-Brucella sera. The in silico analysis of the pan-proteome revealed many homologous proteins, such as Omp16, Omp25, Omp31, SodC, Omp2a, and BhuA. Proteins contained in the vesicles from different Brucella species were detected by anti-Brucella sera. The occurrence of previously described immunogenic proteins derived from OMVs supports the use of these vesicles as candidates to be evaluated as an acellular brucellosis vaccine.
AB - Gram-negative bacteria release nanovesicles, called outer membrane vesicles (OMVs), from their outer membrane. Proteomics has been used to determine their composition. OMVs contain proteins able to elicit an immune response, so they have been proposed as a model to develop acellular vaccines. In this study, OMVs of Brucella suis, B. ovis, B. canis, and B. neotomae were purified and analyzed by SDS-PAGE, transmission electron microscopy and liquid chromatography coupled to mass spectrometry to determine the pan-proteome of these vesicles. In addition, antigenic proteins were detected by western blot with anti-Brucella sera. The in silico analysis of the pan-proteome revealed many homologous proteins, such as Omp16, Omp25, Omp31, SodC, Omp2a, and BhuA. Proteins contained in the vesicles from different Brucella species were detected by anti-Brucella sera. The occurrence of previously described immunogenic proteins derived from OMVs supports the use of these vesicles as candidates to be evaluated as an acellular brucellosis vaccine.
KW - Acellular vaccines
KW - Bacterial vesicles
KW - Brucellosis
KW - Outer membrane vesicles
UR - http://www.scopus.com/inward/record.url?scp=85099080794&partnerID=8YFLogxK
U2 - 10.1007/s00203-020-02170-w
DO - 10.1007/s00203-020-02170-w
M3 - Artículo
C2 - 33432377
AN - SCOPUS:85099080794
VL - 203
SP - 1611
EP - 1626
JO - Archives of Microbiology
JF - Archives of Microbiology
SN - 0302-8933
IS - 4
ER -
