TY - JOUR
T1 - Comparative proteome analysis of Brucella abortus 2308 and its virB type IV secretion system mutant reveals new T4SS-related candidate proteins
AU - Paredes-Cervantes, Vladimir
AU - Flores-Mejía, Raúl
AU - Moreno-Lafont, Martha C.
AU - Lanz-Mendoza, Humberto
AU - Tello-López, Ángel T.
AU - Castillo-Vera, Jane
AU - Pando-Robles, Victoria
AU - Hurtado-Sil, Gerardo
AU - González-González, Edith
AU - Rodríguez-Cortés, Octavio
AU - Gutiérrez-Hoya, Adriana
AU - Vega-Ramírez, María T.
AU - López-Santiago, Rubén
N1 - Funding Information:
This work was supported by Consejo Nacional de Ciencia y Tecnología, CONACyT , México ( 44837Q ), and from Secretaría de Posgrado e Investigación, I.P.N. , México ( 20101365 ). VPC was CONACyT fellow ( 105119 ). MML and RLS are EDI and COFAA fellows. MML and HLM are SNI fellows.
PY - 2011/11/18
Y1 - 2011/11/18
N2 - Brucella abortus is an alpha-2 proteobacteria with a type IV secretion system (T4SS) known as virB, which is necessary to gain virulence by building up a replicative vacuole associated with the endoplasmic reticulum of the host cell. A virB T4SS mutant of the B. abortus 2308 strain and its wild-type strain were grown in acid medium in order to obtain and analyze their proteomes, looking for putative proteins that may serve as T4SS substrates and those that may be subjected to T4SS regulation. A total of 47 overexpressed and 22 underexpressed proteins from the virB T4SS mutant strain were selected and sequenced. Some of the 69 analyzed proteins have not been described before either as over or under-expressed in relation to a virB T4SS mutation, whereas some of them have been already described by other groups as potentially important secretory proteins in other Brucella species. An important number of the proteins identified are outer membrane and periplasmic space protein, which makes them become particularly important new T4SS-related candidate proteins.
AB - Brucella abortus is an alpha-2 proteobacteria with a type IV secretion system (T4SS) known as virB, which is necessary to gain virulence by building up a replicative vacuole associated with the endoplasmic reticulum of the host cell. A virB T4SS mutant of the B. abortus 2308 strain and its wild-type strain were grown in acid medium in order to obtain and analyze their proteomes, looking for putative proteins that may serve as T4SS substrates and those that may be subjected to T4SS regulation. A total of 47 overexpressed and 22 underexpressed proteins from the virB T4SS mutant strain were selected and sequenced. Some of the 69 analyzed proteins have not been described before either as over or under-expressed in relation to a virB T4SS mutation, whereas some of them have been already described by other groups as potentially important secretory proteins in other Brucella species. An important number of the proteins identified are outer membrane and periplasmic space protein, which makes them become particularly important new T4SS-related candidate proteins.
KW - Bioinformatics
KW - Brucella
KW - Proteome
KW - Type IV secretion system
UR - http://www.scopus.com/inward/record.url?scp=81055156127&partnerID=8YFLogxK
U2 - 10.1016/j.jprot.2011.07.020
DO - 10.1016/j.jprot.2011.07.020
M3 - Artículo
C2 - 21875698
SN - 1874-3919
VL - 74
SP - 2959
EP - 2971
JO - Journal of Proteomics
JF - Journal of Proteomics
IS - 12
ER -