Commensal and virulent Escherichia coli strains of vaginal origin are reservoirs of resistance cassettes in class 1 integrons

Manalli Itzahaya Blancarte–Lagunas, Graciela Castro-Escarpulli, Armando Navarro-Ocaña, Gabriela Ibáñez-Cervantes, Laura Margarita Marquez-Valdelamar, Jose Misael Hernández-Carrillo, Juana Salazar-Salinas, Omar Fernando Mendoza-Vásquez, Gabriel Dámazo-Hernández, Oscar Sosa-Hernández, Gregorio León-García, Monica Alethia Cureño-Díaz, Juan Manuel Bello–López

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    Abstract

    Introduction: Antimicrobial resistance in Escherichia coli, one of the causal agents of aerobic vaginitis, leads to the persistence of the infection. The investigation of integrons acquires relevance, since they are elements that are responsible for the acquisition of resistance to antibiotics. The aim of this work was to describe the structural diversity of class 1 integrons in virulent and commensal strains of E. coli isolated from patients with vaginal infection. Methodology: Ninety-two strains of E. coli were isolated from patients with aerobic vaginitis. Resistance profile against 19 antibiotics and class 1 integrons were detected by PCR. The identity and arrangement of cassettes was determined by sequencing. ERIC-PCR assays were carried out in strains with identical arrays. Finally, genotyping by Clermont algorithm and serotyping were performed. Seventeen strains showed integrons located in plasmids. Results: Ten different gene cassette arrays were identified in 30 strains of E. coli. Cassettes corresponding to genes coding for adenylyltransferases (aadA), dihydrofolate reductases (dfrA), and oxacillinases (blaOXA) were detected. Array dfrA17-aadA5 was predominantly prevalent over the other arrays identified. Phylogenetic group A was the most predominant, followed by group B2 and D. Conclusions: This study demonstrates the presence of E. coli of vaginal origin carrying class 1 integrons, which are main genetic elements of capture of resistance genes, with the possibility of capturing new resistance cassettes. These evidences should serve for the modification of protocols in the diagnosis and treatment of aerobic vaginitis, and the development of policies for the rational use of antimicrobials.

    Original languageEnglish
    Pages (from-to)48-58
    Number of pages11
    JournalJournal of Infection in Developing Countries
    Volume14
    Issue number1
    DOIs
    StatePublished - 1 Jan 2020

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    Keywords

    • Antimicrobial resistance
    • Class 1 integrons
    • Escherichia coli

    Cite this

    Blancarte–Lagunas, M. I., Castro-Escarpulli, G., Navarro-Ocaña, A., Ibáñez-Cervantes, G., Marquez-Valdelamar, L. M., Hernández-Carrillo, J. M., Salazar-Salinas, J., Mendoza-Vásquez, O. F., Dámazo-Hernández, G., Sosa-Hernández, O., León-García, G., Cureño-Díaz, M. A., & Bello–López, J. M. (2020). Commensal and virulent Escherichia coli strains of vaginal origin are reservoirs of resistance cassettes in class 1 integrons. Journal of Infection in Developing Countries, 14(1), 48-58. https://doi.org/10.3855/jidc.11940