TY - JOUR
T1 - Combined high-fat diet and sustained high sucrose consumption promotes NAFLD in a murine model
AU - Torres-Villalobos, Gonzalo
AU - Hamdan-Pérez, Nashla
AU - Tovar, Armando R.
AU - Ordaz-Nava, Guillermo
AU - Martínez-Benítez, Braulio
AU - Torre-Villalvazo, Iván
AU - Morán-Ramos, Sofía
AU - Díaz-Villaseñor, Andrea
AU - Noriega, Lilia G.
AU - Hiriart, Marcia
AU - Medina-Santillán, Roberto
AU - Castillo-Hernandez, María Del Carmen
AU - Méndez-Sánchez, Nahum
AU - Uribe, Misael
AU - Torres, Nimbe
N1 - Publisher Copyright:
© 2015, Fundacion Clinica Medica Sur. All rights reserved.
PY - 2015
Y1 - 2015
N2 - Background. The study of NAFLD in humans has several limitations. Using murine models helps to understand disease pathogenesis. Aim. Evaluate the impact of 4 different diets in the production of NAFLD with emphasis on a combined high-fat plus sustained high sucrose consumption. Material and methods. Eight week-old male Wistar rats were divided in four groups and fed for 90 days with the following diets: 1) Control chow diet (C); 2) High-fat cholesterol diet (HFC) + 5% sucrose in drinking water. 3) High-fat cornstarch diet (HFCO) + 5% sucrose in drinking water. 4) Chow diet + 20% sucrose in drinking water (HSD). Metabolic changes, leptin levels, liver histology, hepatic and plasma lipid composition, fasting plasma glucose and insulin and liver gene expression of FAS, SREBP-1 and PPAR-α were evaluated. Results. The HFC diet had the highest grade of steatosis (grade 2 of 3) and HSD showed also steatosis (grade 1). Liver weight TG and cholesterol concentrations in liver were greater in the HFC diet. There were no increased levels of iron in the liver. Rats in HFC gained significantly more weight (P < 0.001). All experimental groups showed fasting hyperglycemia. HFC had the highest glucose level (158.5 ± 7 mg/dL) (P < 0.005). The HSD and the HFCO diets developed also hyperglycemia. HSD had significantly higher fasting hyperinsulinemia. Serum leptin was higher in the HFC diet (p = 0.001). In conclusion, the HFC diet with combination of high fat and high sucrose is more effective in producing NAFLD compared with a high sucrose diet only.
AB - Background. The study of NAFLD in humans has several limitations. Using murine models helps to understand disease pathogenesis. Aim. Evaluate the impact of 4 different diets in the production of NAFLD with emphasis on a combined high-fat plus sustained high sucrose consumption. Material and methods. Eight week-old male Wistar rats were divided in four groups and fed for 90 days with the following diets: 1) Control chow diet (C); 2) High-fat cholesterol diet (HFC) + 5% sucrose in drinking water. 3) High-fat cornstarch diet (HFCO) + 5% sucrose in drinking water. 4) Chow diet + 20% sucrose in drinking water (HSD). Metabolic changes, leptin levels, liver histology, hepatic and plasma lipid composition, fasting plasma glucose and insulin and liver gene expression of FAS, SREBP-1 and PPAR-α were evaluated. Results. The HFC diet had the highest grade of steatosis (grade 2 of 3) and HSD showed also steatosis (grade 1). Liver weight TG and cholesterol concentrations in liver were greater in the HFC diet. There were no increased levels of iron in the liver. Rats in HFC gained significantly more weight (P < 0.001). All experimental groups showed fasting hyperglycemia. HFC had the highest glucose level (158.5 ± 7 mg/dL) (P < 0.005). The HSD and the HFCO diets developed also hyperglycemia. HSD had significantly higher fasting hyperinsulinemia. Serum leptin was higher in the HFC diet (p = 0.001). In conclusion, the HFC diet with combination of high fat and high sucrose is more effective in producing NAFLD compared with a high sucrose diet only.
KW - Animal model
KW - Diet
KW - Nonalcoholic fatty liver disease
KW - Obesity
UR - http://www.scopus.com/inward/record.url?scp=84929574980&partnerID=8YFLogxK
U2 - 10.1016/s1665-2681(19)31176-7
DO - 10.1016/s1665-2681(19)31176-7
M3 - Artículo
SN - 1665-2681
VL - 14
SP - 540
EP - 546
JO - Annals of Hepatology
JF - Annals of Hepatology
IS - 4
ER -