TY - JOUR
T1 - Combination of anti α-Gal-Ppix conjugate and photodynamic therapy for the diagnosis and elimination of HeLa and CaSki cells
AU - Cabrera-Cedillo, Fabiola
AU - Manzo-Sandoval, Anabelle
AU - Medina-Flores, Yolanda
AU - Medina-Escutia, María Edith
AU - Mata-Ruíz, Olga
AU - Cruz-Orea, Alfredo
AU - Garduño-Medina, Jorge Adrián
AU - de la Rosa-Vázquez, Jóse Manuel
AU - Ramón-Gallegos, Eva
N1 - Publisher Copyright:
© 2015 Lifescience Global.
PY - 2015
Y1 - 2015
N2 - The photodynamic therapy (PDT) is a specific and alternative treatment for premalignant and malignant diseases. The α-Gal epitope is expressed in red blood cells and nucleated cells of placental mammals not primates, but absent in old world monkeys, apes and humans. α-Gal epitope has been found in cervical biopsies HPV positive, in biopsies at different stages of cervical intraepithelial neoplasia and in cell lines such as HeLa and CaSki. The aim of this work was to obtain IgG anti α-Gal monoclonal antibody (mAb) and evaluate its usefulness in the photodynamic therapy in order to diagnosis and eliminate infected cells by HPV, types 16 and 18. From the 4C1F6D5G7B8 hybridoma line was obtained a producer hybridoma of IgG3, the mAb was conjugated with PpIX (IgG-PpIX). The IgG-PpIX conjugated was capable of identify the α-Gal epitope present at the membrane of HeLa and CaSki cell lines by immunocitochemistry. The cell lines were exposed to IgG-PpIX and irradiated with a LED system at 635 nm at an energy dose of 64.3 J/cm2. The PDT using IgG anti α-Gal monoclonal antibody has effect on the viability of cervical carcinoma cells; the mortality results obtained were, in descendent order, HeLa (66.9 %) and CaSki (50.8 %). It was possible to conclude that PDT using the immunoconjugate IgG anti α-Gal-PpIX is effective to diagnosis and eliminate cervical carcinoma cells.
AB - The photodynamic therapy (PDT) is a specific and alternative treatment for premalignant and malignant diseases. The α-Gal epitope is expressed in red blood cells and nucleated cells of placental mammals not primates, but absent in old world monkeys, apes and humans. α-Gal epitope has been found in cervical biopsies HPV positive, in biopsies at different stages of cervical intraepithelial neoplasia and in cell lines such as HeLa and CaSki. The aim of this work was to obtain IgG anti α-Gal monoclonal antibody (mAb) and evaluate its usefulness in the photodynamic therapy in order to diagnosis and eliminate infected cells by HPV, types 16 and 18. From the 4C1F6D5G7B8 hybridoma line was obtained a producer hybridoma of IgG3, the mAb was conjugated with PpIX (IgG-PpIX). The IgG-PpIX conjugated was capable of identify the α-Gal epitope present at the membrane of HeLa and CaSki cell lines by immunocitochemistry. The cell lines were exposed to IgG-PpIX and irradiated with a LED system at 635 nm at an energy dose of 64.3 J/cm2. The PDT using IgG anti α-Gal monoclonal antibody has effect on the viability of cervical carcinoma cells; the mortality results obtained were, in descendent order, HeLa (66.9 %) and CaSki (50.8 %). It was possible to conclude that PDT using the immunoconjugate IgG anti α-Gal-PpIX is effective to diagnosis and eliminate cervical carcinoma cells.
KW - Human papilloma virus
KW - IgG
KW - LED system
KW - Monoclonal antibody
KW - Photodynamic therapy
UR - http://www.scopus.com/inward/record.url?scp=84949814738&partnerID=8YFLogxK
U2 - 10.6000/1927-7229.2015.04.03.3
DO - 10.6000/1927-7229.2015.04.03.3
M3 - Artículo
SN - 1927-7210
VL - 4
SP - 106
EP - 112
JO - Journal of Analytical Oncology
JF - Journal of Analytical Oncology
IS - 3
ER -