TY - JOUR
T1 - Colloidal chitin stained with Remazol Brilliant Blue R®, a useful substrate to select chitinolytic microorganisms and to evaluate chitinases
AU - Gómez Ramírez, M.
AU - Rojas Avelizapa, L. I.
AU - Rojas Avelizapa, N. G.
AU - Cruz Camarillo, R.
N1 - Funding Information:
This work was supported in part by the project DEPI-20020611 from CGEPI-IPN, México and project CONACYT-J34997-B, México. We are grateful to COFFA-IPN, México.
PY - 2004/2
Y1 - 2004/2
N2 - A simple and sensitive method based on the use of colloidal chitin stained with Remazol Brilliant Blue R® (RBB) is proposed to evaluate chitinase activity. If this colloidal-stained substrate is included as a carbon source in a liquid medium, this technique allows the selection or the comparison of chitinolytic microorganisms. The colloidal substrate is proportionally solubilized and the dye released is spectrophotometrically quantified at 595 nm. The procedures used for the staining and fixing of RBB in the colloidal chitin, and a comparison with the commercial substrate chitin-azure®, are presented. The influence of several physicochemical and enzymatic parameters on the release of dyes is also shown. Both stained substrates were used for studying the effect of pH, substrate concentration, temperature and time on the chitinase reaction of Bacillus thuringiensis Bt-107.
AB - A simple and sensitive method based on the use of colloidal chitin stained with Remazol Brilliant Blue R® (RBB) is proposed to evaluate chitinase activity. If this colloidal-stained substrate is included as a carbon source in a liquid medium, this technique allows the selection or the comparison of chitinolytic microorganisms. The colloidal substrate is proportionally solubilized and the dye released is spectrophotometrically quantified at 595 nm. The procedures used for the staining and fixing of RBB in the colloidal chitin, and a comparison with the commercial substrate chitin-azure®, are presented. The influence of several physicochemical and enzymatic parameters on the release of dyes is also shown. Both stained substrates were used for studying the effect of pH, substrate concentration, temperature and time on the chitinase reaction of Bacillus thuringiensis Bt-107.
KW - Bacillus thuringiensis
KW - Chitin
KW - Chitinase
KW - Dyed colloidal chitin
KW - Remazol Brilliant Blue-R®
KW - Serratia marcescens
UR - http://www.scopus.com/inward/record.url?scp=1642441679&partnerID=8YFLogxK
U2 - 10.1016/j.mimet.2003.10.011
DO - 10.1016/j.mimet.2003.10.011
M3 - Artículo
SN - 0167-7012
VL - 56
SP - 213
EP - 219
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
IS - 2
ER -