TY - JOUR
T1 - Colletotrichum brevisporum causing anthracnose of papaya (Carica papaya L.) in Mexico
AU - Pacheco-Esteva, Mary Carmen
AU - Soto- Castro, Delia
AU - Vásquez- López, Alfonso
AU - Tovar-Pedraza, Juan Manuel
N1 - Publisher Copyright:
© 2023 The Canadian Phytopathological Society.
PY - 2023
Y1 - 2023
N2 - Papaya fruit cv. Maradol showing typical symptoms of anthracnose were observed in a commercial orchard located in the municipality of Pinotepa Nacional at the coastal region of Oaxaca, Mexico in September 2020. The incidence rate of anthracnose reached 60%. The symptoms consisted of sunken, water-soaked lesions with salmon-coloured conidia masses on the surface of papaya fruit. The aim of this study was to identify the causal agent of papaya anthracnose based on the phylogenetic analysis of four concatenated datasets. The isolate exhibited sparse white to greyish aerial mycelium and clearly shaped concentric rings with exudate of orange conidia on potato dextrose agar. The fungus was identified as Colletotrichum brevisporum using sequence data of the internal transcribed spacer (ITS) region, and part of the actin (act), glyceraldehyde-3-phosphate dehydrogenase (gapdh), and chitin synthase (chs-1) genes. The fungus isolated from diseased fruit produced lesions on wounded fruit and unwounded fruit. The pathogen was re-isolated from lesions on inoculated fruit to fulfill Koch’s postulates. To our knowledge, this is the first report of C. brevisporum causing anthracnose disease on papaya fruit in Mexico.
AB - Papaya fruit cv. Maradol showing typical symptoms of anthracnose were observed in a commercial orchard located in the municipality of Pinotepa Nacional at the coastal region of Oaxaca, Mexico in September 2020. The incidence rate of anthracnose reached 60%. The symptoms consisted of sunken, water-soaked lesions with salmon-coloured conidia masses on the surface of papaya fruit. The aim of this study was to identify the causal agent of papaya anthracnose based on the phylogenetic analysis of four concatenated datasets. The isolate exhibited sparse white to greyish aerial mycelium and clearly shaped concentric rings with exudate of orange conidia on potato dextrose agar. The fungus was identified as Colletotrichum brevisporum using sequence data of the internal transcribed spacer (ITS) region, and part of the actin (act), glyceraldehyde-3-phosphate dehydrogenase (gapdh), and chitin synthase (chs-1) genes. The fungus isolated from diseased fruit produced lesions on wounded fruit and unwounded fruit. The pathogen was re-isolated from lesions on inoculated fruit to fulfill Koch’s postulates. To our knowledge, this is the first report of C. brevisporum causing anthracnose disease on papaya fruit in Mexico.
KW - Colletotrichum brevisporum
KW - anthracnose
KW - papaya
KW - pathogenicity
KW - phylogeny
UR - http://www.scopus.com/inward/record.url?scp=85153054383&partnerID=8YFLogxK
U2 - 10.1080/07060661.2023.2195825
DO - 10.1080/07060661.2023.2195825
M3 - Comentario/Debate
AN - SCOPUS:85153054383
SN - 0706-0661
VL - 45
SP - 360
EP - 366
JO - Canadian Journal of Plant Pathology
JF - Canadian Journal of Plant Pathology
IS - 4
ER -