Clastogenic activity of the new anticonvulsant 4-hydroxy-4-ethyl-4-phenylbutyramide in a Tradescantia bioassay

E. Ruiz, E. Madrigal-Bujaidar, M. Salazar, G. Carvajal, G. Chamorro

Research output: Contribution to journalArticle

Abstract

The compound 4-hydroxy-4-ethyl-4-phenylbutyramide (HEPB) is a cyclic hydroxylamide chemical recently synthesized as part of a series of phenyl alcohol amides with anticonvulsant activity. Experimental studies have shown that HEPB protects rats and mice against several potent convulsive agents. Therefore, studies are underway to determine the probable toxicity of the compound. In this experiment, we used the Tradescantia bioassay to determine whether or not the chemical could increase the level of micronucleated cells in the flower buds. There was a significant increase in micronuclei only with 7 mg/ml, after 24 h of exposure, but not with 1 mg/ml or 5 mg/ml. Higher doses produced cell toxicity. We conclude that HEPB possesses both genotoxic and cytotoxic properties under the experimental conditions used. Thus further genotoxic studies, including a mammal as a model organism, are recommended.
Original languageAmerican English
Pages (from-to)45-47
Number of pages40
JournalMedical Science Research
StatePublished - 6 Feb 1999

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Tradescantia
Bioassay
Biological Assay
Anticonvulsants
Toxicity
Mammals
Amides
Rats
Alcohols
phenylbutyramide
Experiments

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title = "Clastogenic activity of the new anticonvulsant 4-hydroxy-4-ethyl-4-phenylbutyramide in a Tradescantia bioassay",
abstract = "The compound 4-hydroxy-4-ethyl-4-phenylbutyramide (HEPB) is a cyclic hydroxylamide chemical recently synthesized as part of a series of phenyl alcohol amides with anticonvulsant activity. Experimental studies have shown that HEPB protects rats and mice against several potent convulsive agents. Therefore, studies are underway to determine the probable toxicity of the compound. In this experiment, we used the Tradescantia bioassay to determine whether or not the chemical could increase the level of micronucleated cells in the flower buds. There was a significant increase in micronuclei only with 7 mg/ml, after 24 h of exposure, but not with 1 mg/ml or 5 mg/ml. Higher doses produced cell toxicity. We conclude that HEPB possesses both genotoxic and cytotoxic properties under the experimental conditions used. Thus further genotoxic studies, including a mammal as a model organism, are recommended.",
author = "E. Ruiz and E. Madrigal-Bujaidar and M. Salazar and G. Carvajal and G. Chamorro",
year = "1999",
month = "2",
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language = "American English",
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Clastogenic activity of the new anticonvulsant 4-hydroxy-4-ethyl-4-phenylbutyramide in a Tradescantia bioassay. / Ruiz, E.; Madrigal-Bujaidar, E.; Salazar, M.; Carvajal, G.; Chamorro, G.

In: Medical Science Research, 06.02.1999, p. 45-47.

Research output: Contribution to journalArticle

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T1 - Clastogenic activity of the new anticonvulsant 4-hydroxy-4-ethyl-4-phenylbutyramide in a Tradescantia bioassay

AU - Ruiz, E.

AU - Madrigal-Bujaidar, E.

AU - Salazar, M.

AU - Carvajal, G.

AU - Chamorro, G.

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AB - The compound 4-hydroxy-4-ethyl-4-phenylbutyramide (HEPB) is a cyclic hydroxylamide chemical recently synthesized as part of a series of phenyl alcohol amides with anticonvulsant activity. Experimental studies have shown that HEPB protects rats and mice against several potent convulsive agents. Therefore, studies are underway to determine the probable toxicity of the compound. In this experiment, we used the Tradescantia bioassay to determine whether or not the chemical could increase the level of micronucleated cells in the flower buds. There was a significant increase in micronuclei only with 7 mg/ml, after 24 h of exposure, but not with 1 mg/ml or 5 mg/ml. Higher doses produced cell toxicity. We conclude that HEPB possesses both genotoxic and cytotoxic properties under the experimental conditions used. Thus further genotoxic studies, including a mammal as a model organism, are recommended.

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