TY - JOUR
T1 - Chemopreventive and antioxidant effect of polyphenol free Spirulina maxima and its hydrolyzed protein content
T2 - Investigation on azoxymethane treated mice
AU - Martinez-Palma, Nikte Y.
AU - Dávila-Ortiz, Gloria
AU - Jiménez-Martínez, Cristian
AU - Madrigal-Bujaidar, Eduardo
AU - Álvarez-González, Isela
N1 - Publisher Copyright:
© 2017 Pharmacognosy Magazine.
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Background: Spirulina maxima (Sm) is known to have nutritive value as well as a number of potentially useful biomedical properties. Objectives: The initial purpose of this report was to evaluate the inhibitory effect of the alga (without its polyphenol content), on the induction of azoxymethane (AOM)-induced colon aberrant crypts (AC) in mouse. Besides, we hydrolyzed the protein content of such mixture. Our second aim was to determine the inhibitory potential of this last plant mixture on the AOM-induced colon AC in mouse. Moreover, we also determined the effect of the two indicated Sm samples on the oxidative damage caused by AOM in the colon and liver of treated mice. Materials and Methods: The experiment lasted 5 weeks. At the end, we registered the level of AC, nitric oxide, and the lipid and protein oxidation. Results: Our results showed the following: (1) the carcinogen increased more than 18 times the amount of the AC found in the control group. (2) On the contrary, the two tested mixtures of Sm produced a significant reduction over this damage (about 45%). (3) The two tested Sm mixtures were generally able to reduce the oxidative stress markers although with variable effects which go from 59% to 100% with respect to the control mice. Conclusion: Therefore, the present report established that the tested Sm fractions have mouse colon anticarcinogenic potential, partially related with their antioxidant capacity. Our report also suggested the need to further evaluate specific Sm chemicals as chemopreventive agents.
AB - Background: Spirulina maxima (Sm) is known to have nutritive value as well as a number of potentially useful biomedical properties. Objectives: The initial purpose of this report was to evaluate the inhibitory effect of the alga (without its polyphenol content), on the induction of azoxymethane (AOM)-induced colon aberrant crypts (AC) in mouse. Besides, we hydrolyzed the protein content of such mixture. Our second aim was to determine the inhibitory potential of this last plant mixture on the AOM-induced colon AC in mouse. Moreover, we also determined the effect of the two indicated Sm samples on the oxidative damage caused by AOM in the colon and liver of treated mice. Materials and Methods: The experiment lasted 5 weeks. At the end, we registered the level of AC, nitric oxide, and the lipid and protein oxidation. Results: Our results showed the following: (1) the carcinogen increased more than 18 times the amount of the AC found in the control group. (2) On the contrary, the two tested mixtures of Sm produced a significant reduction over this damage (about 45%). (3) The two tested Sm mixtures were generally able to reduce the oxidative stress markers although with variable effects which go from 59% to 100% with respect to the control mice. Conclusion: Therefore, the present report established that the tested Sm fractions have mouse colon anticarcinogenic potential, partially related with their antioxidant capacity. Our report also suggested the need to further evaluate specific Sm chemicals as chemopreventive agents.
KW - Antioxidation
KW - Chemoprevention
KW - Mouse
KW - Spirulina maxima fractions
UR - http://www.scopus.com/inward/record.url?scp=85032990512&partnerID=8YFLogxK
U2 - 10.4103/0973-1296.210197
DO - 10.4103/0973-1296.210197
M3 - Artículo
C2 - 28808375
SN - 0973-1296
VL - 13
SP - S164-S169
JO - Pharmacognosy Magazine
JF - Pharmacognosy Magazine
IS - 50
ER -