TY - JOUR
T1 - Caracterización del proceso biológico de sulfuro oxidación
T2 - un enfoque de respirometría de pulsos in situ y microrespirometria de pulsos ex situ
AU - Keb-Fonseca, G. A.
AU - Guerrero-Barajas, C.
AU - Ordaz, A.
N1 - Publisher Copyright:
© 2022, Universidad Autonoma Metropolitana. All rights reserved.
PY - 2022/1/1
Y1 - 2022/1/1
N2 - During the optimization of environmental biotechnology processes, it is important to count on proper and reliable information about the kinetic and stoichiometric parameters of the microorganisms. In this work, the biological sulfide oxidation process was assessed under two novel dynamic pulse respirometric approaches: in situ pulse respirometry conducted in airlift bioreactor and ex situ pulse respirometry carried out in microreactors (microrespirometry) with samples taken from the airlift bioreactor. The process was characterized in terms of the growth yield, substrate oxidation yield, maximum oxygen uptake rate, affinity constant, and mass transfer coefficient. The in situ pulse respirometry showed to be a reproducible technique that allowed the determination of the kinetic and stoichiometric parameters besides the detection of mass transfer limitations in the airlift bioreactor, however its use in biological sulfide oxidation is limited to a few experiments and experimental conditions with significant time investment. On the other hand, the ex situ pulse microrespirometry allowed the acquisition of a higher amount of information under a broader range of sulfide concentrations (from 5 to 60 mg H2S L-1) and experimental conditions such as different pH values. The results obtained showed that the ex situ microrespirometry technique would be preferable over in situ pulse respirometry for the proper and reliable characterization of the sulfide oxidation process.
AB - During the optimization of environmental biotechnology processes, it is important to count on proper and reliable information about the kinetic and stoichiometric parameters of the microorganisms. In this work, the biological sulfide oxidation process was assessed under two novel dynamic pulse respirometric approaches: in situ pulse respirometry conducted in airlift bioreactor and ex situ pulse respirometry carried out in microreactors (microrespirometry) with samples taken from the airlift bioreactor. The process was characterized in terms of the growth yield, substrate oxidation yield, maximum oxygen uptake rate, affinity constant, and mass transfer coefficient. The in situ pulse respirometry showed to be a reproducible technique that allowed the determination of the kinetic and stoichiometric parameters besides the detection of mass transfer limitations in the airlift bioreactor, however its use in biological sulfide oxidation is limited to a few experiments and experimental conditions with significant time investment. On the other hand, the ex situ pulse microrespirometry allowed the acquisition of a higher amount of information under a broader range of sulfide concentrations (from 5 to 60 mg H2S L-1) and experimental conditions such as different pH values. The results obtained showed that the ex situ microrespirometry technique would be preferable over in situ pulse respirometry for the proper and reliable characterization of the sulfide oxidation process.
KW - Airlift bioreactor
KW - Inhibition models
KW - Microreactor
KW - Pulse microrespirometry
KW - Sulfide oxidation
UR - http://www.scopus.com/inward/record.url?scp=85122665685&partnerID=8YFLogxK
U2 - 10.24275/rmiq/IA2575
DO - 10.24275/rmiq/IA2575
M3 - Artículo
AN - SCOPUS:85122665685
SN - 1665-2738
VL - 21
JO - Revista Mexicana de Ingeniera Quimica
JF - Revista Mexicana de Ingeniera Quimica
IS - 1
M1 - IA2575
ER -