Characterization of the 5' subtilisin (aprE) regulatory region from Bacillus subtilis

Janet Jan; Fernando Valle; Francisco Bolivar; Enrique Merino

doi:10.1016/S0378-1097(99)00621-7

Characterization of the 5' subtilisin (aprE) regulatory region from Bacillus subtilis

Janet Jan, Fernando Valle, Francisco Bolivar, Enrique Merino

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The aprE gene of Bacillus subtilis encodes the major serine alkaline protease known as subtilisin. It is expressed during the transition state and transcribed by the σ(A) form of the RNA polymerase (RNAP). In this work, we characterized the regulatory region of the aprE gene (rraprE) from B. subtilis. By computer analysis and site-directed mutagenesis, we localized the aprE promoter sequence 7 bp upstream from its transcription initiation site (TIS). We also characterized the static curvature properties of the rraprE DNA and found two different areas of DNA bending, within the first 400 bp upstream of its TIS. We postulate that these particular curved DNA regions could play a role in the interaction with some regulatory proteins and discuss possible implications related to aprE transcription regulation. (C) Federation of European Microbiological Societies.

Original language	English
Pages (from-to)	9-14
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	183
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1097(99)00621-7
State	Published - 1 Feb 2000
Externally published	Yes

Keywords

Bacillus subtilis
Curved DNA
Promoter sequence
Site-directed mutagenesis
aprE

Access to Document

10.1016/S0378-1097(99)00621-7

Cite this

@article{619dc29d20334fdab44a6e55fb04ffc9,

title = "Characterization of the 5' subtilisin (aprE) regulatory region from Bacillus subtilis",

abstract = "The aprE gene of Bacillus subtilis encodes the major serine alkaline protease known as subtilisin. It is expressed during the transition state and transcribed by the σ(A) form of the RNA polymerase (RNAP). In this work, we characterized the regulatory region of the aprE gene (rraprE) from B. subtilis. By computer analysis and site-directed mutagenesis, we localized the aprE promoter sequence 7 bp upstream from its transcription initiation site (TIS). We also characterized the static curvature properties of the rraprE DNA and found two different areas of DNA bending, within the first 400 bp upstream of its TIS. We postulate that these particular curved DNA regions could play a role in the interaction with some regulatory proteins and discuss possible implications related to aprE transcription regulation. (C) Federation of European Microbiological Societies.",

keywords = "Bacillus subtilis, Curved DNA, Promoter sequence, Site-directed mutagenesis, aprE",

author = "Janet Jan and Fernando Valle and Francisco Bolivar and Enrique Merino",

note = "Funding Information: We thank Edmundo Calva, Guillermo Gosset and Beatr{\'ı}z Palmeros for critical reading of the manuscript and Mercedes Enzaldo for her excellent technical assistance. Janet Jan was supported by scholarship from Consejo Nacional de Ciencia y Tecnolog{\'ı}a (CONACyT) M{\'e}xico and Direcci{\'o}n General de Estudios de Posgrado (DGEP) UNAM-M{\'e}xico.",

year = "2000",

month = feb,

day = "1",

doi = "10.1016/S0378-1097(99)00621-7",

language = "Ingl{\'e}s",

volume = "183",

pages = "9--14",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

number = "1",

}

TY - JOUR

T1 - Characterization of the 5' subtilisin (aprE) regulatory region from Bacillus subtilis

AU - Jan, Janet

AU - Valle, Fernando

AU - Bolivar, Francisco

AU - Merino, Enrique

N1 - Funding Information: We thank Edmundo Calva, Guillermo Gosset and Beatrı́z Palmeros for critical reading of the manuscript and Mercedes Enzaldo for her excellent technical assistance. Janet Jan was supported by scholarship from Consejo Nacional de Ciencia y Tecnologı́a (CONACyT) México and Dirección General de Estudios de Posgrado (DGEP) UNAM-México.

PY - 2000/2/1

Y1 - 2000/2/1

N2 - The aprE gene of Bacillus subtilis encodes the major serine alkaline protease known as subtilisin. It is expressed during the transition state and transcribed by the σ(A) form of the RNA polymerase (RNAP). In this work, we characterized the regulatory region of the aprE gene (rraprE) from B. subtilis. By computer analysis and site-directed mutagenesis, we localized the aprE promoter sequence 7 bp upstream from its transcription initiation site (TIS). We also characterized the static curvature properties of the rraprE DNA and found two different areas of DNA bending, within the first 400 bp upstream of its TIS. We postulate that these particular curved DNA regions could play a role in the interaction with some regulatory proteins and discuss possible implications related to aprE transcription regulation. (C) Federation of European Microbiological Societies.

AB - The aprE gene of Bacillus subtilis encodes the major serine alkaline protease known as subtilisin. It is expressed during the transition state and transcribed by the σ(A) form of the RNA polymerase (RNAP). In this work, we characterized the regulatory region of the aprE gene (rraprE) from B. subtilis. By computer analysis and site-directed mutagenesis, we localized the aprE promoter sequence 7 bp upstream from its transcription initiation site (TIS). We also characterized the static curvature properties of the rraprE DNA and found two different areas of DNA bending, within the first 400 bp upstream of its TIS. We postulate that these particular curved DNA regions could play a role in the interaction with some regulatory proteins and discuss possible implications related to aprE transcription regulation. (C) Federation of European Microbiological Societies.

KW - Bacillus subtilis

KW - Curved DNA

KW - Promoter sequence

KW - Site-directed mutagenesis

KW - aprE

UR - http://www.scopus.com/inward/record.url?scp=0033955535&partnerID=8YFLogxK

U2 - 10.1016/S0378-1097(99)00621-7

DO - 10.1016/S0378-1097(99)00621-7

M3 - Artículo

SN - 0378-1097

VL - 183

SP - 9

EP - 14

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Characterization of the 5' subtilisin (aprE) regulatory region from Bacillus subtilis

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this