TY - JOUR
T1 - Characterization of Pleurotus djamor neohaplonts recovered by production of protoplasts and chemical dedikaryotization
AU - Hernández, Abraham Sánchez
AU - Valenzuela Cobos, Juan Diego
AU - Martínez, Joel Herrera
AU - Arce, Ramón Villanueva
AU - de las Mercedes Gómez y Gomez, Yolanda
AU - Segura, Paola Berenice Zarate
AU - Aguilar, María Eugenia Garín
AU - Lara, Hermilo Leal
AU - del Toro, Gustavo Valencia
N1 - Publisher Copyright:
© 2019, King Abdulaziz City for Science and Technology.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Production of hybrid strains is accomplished by mating monosporic isolates or neohaplonts, obtained either by chemical dedikaryotization or by production of protoplast. However, differences in growth rate among recovered neohaplonts have been reported. The presence of phenotypic and genetic changes among the neohaplonts recovered either by chemical dedikaryotization or by production of protoplast, was evaluated by measuring growth and morphology, and by molecular characterization using six ISSR markers to identify polymorphisms. Neohaplonts recovered by both methods presented variation in growth rate depending on their compatibility type and recovery method. Using ISSR markers, 59.2% polymorphism was established. Neohaplonts recovered by both monokaryotization procedures presented differences in growth rate and polymorphism.
AB - Production of hybrid strains is accomplished by mating monosporic isolates or neohaplonts, obtained either by chemical dedikaryotization or by production of protoplast. However, differences in growth rate among recovered neohaplonts have been reported. The presence of phenotypic and genetic changes among the neohaplonts recovered either by chemical dedikaryotization or by production of protoplast, was evaluated by measuring growth and morphology, and by molecular characterization using six ISSR markers to identify polymorphisms. Neohaplonts recovered by both methods presented variation in growth rate depending on their compatibility type and recovery method. Using ISSR markers, 59.2% polymorphism was established. Neohaplonts recovered by both monokaryotization procedures presented differences in growth rate and polymorphism.
KW - Hybrid production
KW - ISSR
KW - Monokaryotization
KW - Polymorphism
UR - http://www.scopus.com/inward/record.url?scp=85059344599&partnerID=8YFLogxK
U2 - 10.1007/s13205-018-1532-4
DO - 10.1007/s13205-018-1532-4
M3 - Artículo
C2 - 30622862
SN - 2190-572X
VL - 9
JO - 3 Biotech
JF - 3 Biotech
IS - 1
M1 - 24
ER -