Characterization of IS1389, a new member of the IS3 family of insertion sequences isolated from Xanthomonas campestris pv. amaranthicola

Pilar Gómez; Rosa M. Ribas-Aparicio; Ana I. Pélaez; M. Rosario Rodicio

doi:10.1007/s002030050734

Characterization of IS1389, a new member of the IS3 family of insertion sequences isolated from Xanthomonas campestris pv. amaranthicola

Pilar Gómez, Rosa M. Ribas-Aparicio, Ana I. Pélaez, M. Rosario Rodicio

Escuela Nacional de Ciencias Biológicas (ENCB)

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

IS1389, a new insertion sequence belonging to the IS3 family, has been identified in Xanthomonas campestris pv. amaranthicola. The genome of this bacterium contains at least 11 copies of the element, whereas no hybridizing sequences were detected in other Xanthomonas species [X. axonopodis, X. fragaridae, X. phaseoli, and X. (Stenotrophomonas) maltophila]. Two nearly identical copies of the element (IS1389-A and IS1389-B) were characterized. According to analysis of sequence alignments and similar structural features, IS1389 belongs to the IS407 subgroup of the IS3 family, which duplicates 4bp of target DNA upon insertion. IS1389-A was found in the proximity of the modification gene of the XamI restriction-modification system.

Original language	English
Pages (from-to)	15-21
Number of pages	7
Journal	Archives of Microbiology
Volume	172
Issue number	1
DOIs	https://doi.org/10.1007/s002030050734
State	Published - 1999

Keywords

IS1389
IS3 family
Insertion sequence
XamI restriction-modification system
Xanthomonas campestris pv. amaranthicola

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10.1007/s002030050734

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title = "Characterization of IS1389, a new member of the IS3 family of insertion sequences isolated from Xanthomonas campestris pv. amaranthicola",

abstract = "IS1389, a new insertion sequence belonging to the IS3 family, has been identified in Xanthomonas campestris pv. amaranthicola. The genome of this bacterium contains at least 11 copies of the element, whereas no hybridizing sequences were detected in other Xanthomonas species [X. axonopodis, X. fragaridae, X. phaseoli, and X. (Stenotrophomonas) maltophila]. Two nearly identical copies of the element (IS1389-A and IS1389-B) were characterized. According to analysis of sequence alignments and similar structural features, IS1389 belongs to the IS407 subgroup of the IS3 family, which duplicates 4bp of target DNA upon insertion. IS1389-A was found in the proximity of the modification gene of the XamI restriction-modification system.",

keywords = "IS1389, IS3 family, Insertion sequence, XamI restriction-modification system, Xanthomonas campestris pv. amaranthicola",

author = "Pilar G{\'o}mez and Ribas-Aparicio, {Rosa M.} and P{\'e}laez, {Ana I.} and Rodicio, {M. Rosario}",

note = "Funding Information: Acknowledgements P. G{\'o}mez was the recipient of a grant from the FYCIT (Asturias, Spain), and R. M. Ribas-Aparicio was the recipient of a fellowship from the COFAA-IPN and the Banco de M{\'e}xico (Mexico). This work was supported by grant no. BIO91-0563 (CICYT; Spain).",

year = "1999",

doi = "10.1007/s002030050734",

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volume = "172",

pages = "15--21",

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T1 - Characterization of IS1389, a new member of the IS3 family of insertion sequences isolated from Xanthomonas campestris pv. amaranthicola

AU - Gómez, Pilar

AU - Ribas-Aparicio, Rosa M.

AU - Pélaez, Ana I.

AU - Rodicio, M. Rosario

N1 - Funding Information: Acknowledgements P. Gómez was the recipient of a grant from the FYCIT (Asturias, Spain), and R. M. Ribas-Aparicio was the recipient of a fellowship from the COFAA-IPN and the Banco de México (Mexico). This work was supported by grant no. BIO91-0563 (CICYT; Spain).

PY - 1999

Y1 - 1999

N2 - IS1389, a new insertion sequence belonging to the IS3 family, has been identified in Xanthomonas campestris pv. amaranthicola. The genome of this bacterium contains at least 11 copies of the element, whereas no hybridizing sequences were detected in other Xanthomonas species [X. axonopodis, X. fragaridae, X. phaseoli, and X. (Stenotrophomonas) maltophila]. Two nearly identical copies of the element (IS1389-A and IS1389-B) were characterized. According to analysis of sequence alignments and similar structural features, IS1389 belongs to the IS407 subgroup of the IS3 family, which duplicates 4bp of target DNA upon insertion. IS1389-A was found in the proximity of the modification gene of the XamI restriction-modification system.

AB - IS1389, a new insertion sequence belonging to the IS3 family, has been identified in Xanthomonas campestris pv. amaranthicola. The genome of this bacterium contains at least 11 copies of the element, whereas no hybridizing sequences were detected in other Xanthomonas species [X. axonopodis, X. fragaridae, X. phaseoli, and X. (Stenotrophomonas) maltophila]. Two nearly identical copies of the element (IS1389-A and IS1389-B) were characterized. According to analysis of sequence alignments and similar structural features, IS1389 belongs to the IS407 subgroup of the IS3 family, which duplicates 4bp of target DNA upon insertion. IS1389-A was found in the proximity of the modification gene of the XamI restriction-modification system.

KW - IS1389

KW - IS3 family

KW - Insertion sequence

KW - XamI restriction-modification system

KW - Xanthomonas campestris pv. amaranthicola

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DO - 10.1007/s002030050734

M3 - Artículo

SN - 0302-8933

VL - 172

SP - 15

EP - 21

JO - Archives of Microbiology

JF - Archives of Microbiology

IS - 1

ER -

Characterization of IS1389, a new member of the IS3 family of insertion sequences isolated from Xanthomonas campestris pv. amaranthicola

Abstract

Keywords

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